Aminopolysaccharide-based Personalized Bone Implants Prepared by Direct Low-temperature 3D Printing

abstract

English

The objective of the ProfiBONE project is to establish close multidisciplinary cooperation between Czech and Icelandic partners with a focus on the research and development of biofunctionalized polymer-ceramic ink for low-temperature 3D printing of patient-specific bone implants. The premise is to improve the mechanical, degradation, osteoinductive, and antibacterial properties of bone implants by modifying the ceramic cement with a binder based on biodegradable synthetic polymers (developed at BUT), bioactive aminopolysaccharides (provadvantage of this approach is avoiding the denaturation conditions such as radiation (laser or UV) or (curing of the final product). Therefore, it is possible to add bioactive additives directly to the paste during printing without losing their biological activity. This procedure also guarantees an even distribution of bioactive substances in the implant, which positively affects their release and the complex function of the implant. The parameters of both the printer and the composite paste were optimized for direct 3D printing of samples with different extrusion coefficients, compositions, degradation times, and mechanical properties. The results showed that the use of different internal structures greatly affects not only the resulting mechanical properties but also the suitability for cell seeding, where the original regular structure was inappropriate. The modified structure prevented cells from falling through the scaffolds and ensured the uniform inoculation of the materials with SAOS-2 cells for all types of samples with different internal porosity. The in vitro biocompatibility tests on differentiated bone cells and mesenchymal stem cells are being performed gradually. The osseointegration properties of the printed implants are monitored using an established rat femoral bone defect model and are tested on a new rat parietal bone defect model. The recent histological evaluation confirmed osseointegration of new bone into the printed implant without significant inflammation of surrounding tissue. In vivo resorbability of 3D printed implants in skull defects are being currently studied.

bone resorbable cement, direct low-temperature 3D printing, bioactive substances, SAOS-2 cells, in vivo, femoral bone defect model

VOJTOVÁ, L.; VIŠTEJNOVÁ, L.; ÖRLYGSSON, G.; CHUEN HOW, N.; HLINÁKOVÁ, K.; LYSÁKOVÁ, K.; KADLECOVÁ, Z.; MICHLOVSKÁ, L.; BRTNÍKOVÁ, J.; ŽÍDEK, J.; MENČÍK, P.; KLEIN, P.

13. 9. 2023

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https://esbp2023.com/src/ESBP2023_Book_of_Abstracts.pdf