Publication detail

Antagonistic Interaction of Selenium and Cadmium in Human Hepatic Cells Through Selenoproteins

RAMÍREZ-ACOSTA, S., UHLÍŘOVÁ, R., NAVARRO, F., GÓMEZ-ARIZA, J.L., GARCÍA-BARRERA, T.

Original Title

Antagonistic Interaction of Selenium and Cadmium in Human Hepatic Cells Through Selenoproteins

Type

journal article in Web of Science

Language

English

Original Abstract

Cadmium (Cd) is a highly toxic heavy metal for humans and animals, which is associated with acute hepatotoxicity. Selenium (Se) confers protection against Cd-induced toxicity in cells, diminishing the levels of ROS and increasing the activity of antioxidant selenoproteins such as glutathione peroxidase (GPx). The aim of this study was to evaluate the antagonistic effect of selenomethionine (SeMet) against Cd toxicity in HepG2 cells, through the modulation of selenoproteins. To this end, the cells were cultured in the presence of 100 mu M SeMet and 5 mu M, 15 mu M, and 25 mu M CdCl2 and a combination of both species for 24 h. At the end of the experiment, cell viability was determined by MTT assay. The total metal content of Cd and Se was analyzed by triple-quadrupole inductively coupled plasma-mass spectrometry (ICP-QqQ-MS). To quantify the concentration of three selenoproteins [GPx, selenoprotein P (SELENOP), and selenoalbumin (SeAlb)] and selenometabolites, an analytical methodology based on column switching and a species-unspecific isotopic dilution approach using two-dimensional size exclusion and affinity chromatography coupled to ICP-QqQ-MS was applied. The co-exposure of SeMet and Cd in HepG2 cells enhanced the cell viability and diminished the Cd accumulation in cells. Se supplementation increased the levels of selenometabolites, GPx, SELENOP, and SeAlb; however, the presence of Cd resulted in a significant diminution of selenometabolites and SELENOP. These results suggested that SeMet may affect the accumulation of Cd in cells, as well as the suppression of selenoprotein synthesis induced by Cd.

Keywords

cadmium; selenoprotein; HepG2; ICP-QqQ-MS; column switching; isotopic dilution; selenomethionine

Authors

RAMÍREZ-ACOSTA, S., UHLÍŘOVÁ, R., NAVARRO, F., GÓMEZ-ARIZA, J.L., GARCÍA-BARRERA, T.

Released

25. 5. 2022

Publisher

FRONTIERS MEDIA SA

Location

LAUSANNE

ISBN

2296-2646

Periodical

Frontiers in Chemistry

Year of study

10

Number

5

State

Swiss Confederation

Pages count

8

URL

https://www.frontiersin.org/articles/10.3389/fchem.2022.891933/full

BibTex

@article{BUT182860,
  author="Renata {Uhlířová}",
  title="Antagonistic Interaction of Selenium and Cadmium in Human Hepatic Cells Through Selenoproteins",
  journal="Frontiers in Chemistry",
  year="2022",
  volume="10",
  number="5",
  pages="8",
  doi="10.3389/fchem.2022.891933",
  issn="2296-2646",
  url="https://www.frontiersin.org/articles/10.3389/fchem.2022.891933/full"
}