Publication detail

TRACING NEW POTENTIAL CHEMOTERAPEUTICS USING ICP-MS AND LA-ICP-MS

BILAVČÍKOVÁ, K. VAŠINOVÁ GALIOVÁ, M. COUFALÍK, P.HRSTKA, R. VONKA, P. PINKAS, J. KARBAN, J.

Original Title

Type

conference proceedings

Language

English

Original Abstract

Our research is focused on the investigation of the distribution and efficiency of newly synthesized metallocenes with ruthenium, hafnium or zirconium as a central atom in their structure towards lung cancer cell lines A549 and H1299. The behaviour of these cells was monitored in relation to the presence or absence of the gene Anterior GRadient 2 (AGR2) to investigate its impact on the sensitivity of these cells to administered drugs. Determination of the amount of new potential chemotherapeutic agents present in the cells was performed by inductively coupled plasma mass spectrometry (ICP-MS) and in conjunction with laser ablation (LA-ICP-MS). Metal distributions within the single cells and 2D-imaging was also studied. Based on the experiment conducted, the greatest potential was found for ruthenium-based drugs, which showed the highest permeability, exhibited the highest cytotoxicity, and since entered the cells in the large amount.

Keywords

cancer; metallocenes; LA-ICP-MS; singel-cell analysis

Authors

BILAVČÍKOVÁ, K.; VAŠINOVÁ GALIOVÁ, M.; COUFALÍK, P.HRSTKA, R.; VONKA, P.; PINKAS, J.; KARBAN, J.

Released

4. 9. 2022

ISBN

978-80-88195-41-2

Book

BOOK OF ABSTRACTS ESAS 2022

BibTex

@proceedings{BUT182194,
  editor="BILAVČÍKOVÁ, K. and VAŠINOVÁ GALIOVÁ, M. and COUFALÍK, P.HRSTKA, R. and VONKA, P. and PINKAS, J. and KARBAN, J.",
  title="TRACING NEW POTENTIAL CHEMOTERAPEUTICS USING ICP-MS AND LA-ICP-MS",
  year="2022",
  isbn="978-80-88195-41-2"
}