Publication detail

Promising effect of topical antimicrobial 'biobetters' against methicillin-resistant Staphylococcus aureus strains

RAŠKA, F. LIPOVÝ, B. HOLOUBEK, J. KOBZOVÁ, Š. VACEK, L. VOJTOVÁ, L. JANDA, L.

Original Title

Type

journal article in Web of Science

Language

English

Original Abstract

Successful control of burn wound infection is one of the keystones in the successful treatment of patients with burn injury. The increase in the prevalence of resistant strains of pathogens dramatically reduces the options of antibiotic treatment. Infectious complications caused by methicillin-resistant Staphylococcus aureus (MRSA) are known to be associated with significantly higher mortality than those caused by methicillin-sensitive strains (MSSA; 42 % and 18 %, respectively). This situation underlines the necessity for other strategies to control infectious complications of burn wounds. The use of enzybiotics (such as endolysins and lysostaphins), specific products of bacteriophages or competing bacteria, appears to be a promising alternative approach. Their advantages include high specificity and, in particular, they appear less susceptible to the development of resistant strains of bacteria. The principal limitations of the current options for the use of enzybiotics in the topical treatment of MRSA-induced burn wound infections are 1) low production associated with a high price, 2) short duration of the enzymatic activity, which is caused by the structural instability of enzymes resulting in protein denaturation and 3) low resistance of the enzyme to proteases – in particular, matrix metalloproteinases (MMPs), which are produced in the burn wound area in high amounts. Hence, creating a sufficiently persistent and thermally stable variant of such enzymes in a ‘biobetter’ modification, maintaining the original (wild-type) antimicrobial activity, is the principal goal of researchers that would pave the way for broader use of enzybiotics in clinical practice, especially if such a product would be financially accessible.

Keywords

enzybiotics, lysostaphins, infection, methicillin-resistant Staphylococcus aureus, in vitro, in vivo

Authors

RAŠKA, F.; LIPOVÝ, B.; HOLOUBEK, J.; KOBZOVÁ, Š.; VACEK, L.; VOJTOVÁ, L.; JANDA, L.

Released

26. 7. 2022

Publisher

ELSEVIER SCI LTD

Location

OXFORD

ISBN

1879-1409

Periodical

BURNS

Year of study

Number

State

United Kingdom of Great Britain and Northern Ireland

Pages from

1770

Pages to

1772

Pages count

URL

https://www-sciencedirect-com.ezproxy.lib.vutbr.cz/science/article/pii/S0305417922002212

BibTex

@article{BUT180593,
  author="Filip {Raška} and Břetislav {Lipový} and Jakub {Holoubek} and Šárka {Kobzová} and Lukáš {Vacek} and Lucy {Vojtová} and Lubomír {Janda}",
  title="Promising effect of topical antimicrobial 'biobetters' against methicillin-resistant Staphylococcus aureus strains",
  journal="BURNS",
  year="2022",
  volume="48",
  number="7",
  pages="1770--1772",
  doi="10.1016/j.burns.2022.08.011",
  issn="1879-1409",
  url="https://www-sciencedirect-com.ezproxy.lib.vutbr.cz/science/article/pii/S0305417922002212"
}