Publication detail

Effect of Polymeric Nanoparticles with Entrapped Fish Oil or Mupirocin on Skin Wound Healing Using a Porcine Model

KOMPRDA, T. SLÁDEK, Z. VÍCENOVÁ, M. SIMONOVÁ, J. FRANKE, G. LIPOVÝ, B. MATEJOVIČOVÁ, M. KACVINSKÁ, K. SABLIOV, C. ASTETE, C. LEVÁ, L. POPELKOVÁ, V. BÁTIK, A. VOJTOVÁ, L.

Original Title

Effect of Polymeric Nanoparticles with Entrapped Fish Oil or Mupirocin on Skin Wound Healing Using a Porcine Model

Type

journal article in Web of Science

Language

English

Original Abstract

The utilization of poly(lactic-co-glycolic) acid (PLGA) nanoparticles (NPs) with entrapped fish oil (FO) loaded in collagen-based scaffolds for cutaneous wound healing using a porcine model is unique for the present study. Full-depth cutaneous excisions (5 x 5 cm) on the pig dorsa were treated with pure collagen scaffold (control, C), empty PLGA NPs (NP), FO, mupirocin (MUP), PLGA NPs with entrapped FO (NP/FO) and PLGA NPs with entrapped MUP (NP/MUP). The following markers were evaluated on days 0, 3, 7, 14 and 21 post-excision: collagen, hydroxyproline (HP), angiogenesis and expressions of the COX2, EGF, COL1A1, COL1A3, TGFB1, VEGFA, CCL5 and CCR5 genes. The hypothesis that NP/FO treatment is superior to FO alone and that it is comparable to NP/MUP was tested. NP/FO treatment increased HP in comparison with both FO alone and NP/MUP (day 14) but decreased (p < 0.05) angiogenesis in comparison with FO alone (day 3). NP/FO increased (p < 0.05) the expression of the CCR5 gene (day 3) and tended (p > 0.05) to increase the expressions of the EGF (day 7, day 14), TGFB1 (day 21) and CCL5 (day 7, day 21) genes as compared with NP/MUP. NP/FO can be suggested as a suitable alternative to NP/MUP in cutaneous wound treatment.

Keywords

cutaneous wounds; polyunsaturated fatty acids n-3; nanoparticles; poly(lactic-co-glycolic) acid; mupirocin; collagen; hydroxyproline; cyclooxygenase-2; transforming growth factor

Authors

KOMPRDA, T.; SLÁDEK, Z.; VÍCENOVÁ, M.; SIMONOVÁ, J.; FRANKE, G.; LIPOVÝ, B.; MATEJOVIČOVÁ, M.; KACVINSKÁ, K.; SABLIOV, C.; ASTETE, C.; LEVÁ, L.; POPELKOVÁ, V.; BÁTIK, A.; VOJTOVÁ, L.

Released

11. 7. 2022

Publisher

MDPI

Location

BASEL

ISBN

1422-0067

Periodical

International Journal of Molecular Sciences

Year of study

23

Number

14

State

Swiss Confederation

Pages from

7663

Pages to

7682

Pages count

20

URL

https://www.mdpi.com/1422-0067/23/14/7663

BibTex

@article{BUT180332,
  author="Tomáš {Komprda} and Zbyšek {Sládek} and Monika {Vícenová} and Jana {Simonová} and Gabriela {Franke} and Břetislav {Lipový} and Milena {Matejovičová} and Katarína {Verčimáková} and Cristina {Sabliov} and Carlos E. {Astete} and Lenka {Levá} and Vendula {Popelková} and Andrej {Bátik} and Lucy {Vojtová}",
  title="Effect of Polymeric Nanoparticles with Entrapped Fish Oil or Mupirocin on Skin Wound Healing Using a Porcine Model",
  journal="International Journal of Molecular Sciences",
  year="2022",
  volume="23",
  number="14",
  pages="20",
  doi="10.3390/ijms23147663",
  issn="1422-0067",
  url="https://www.mdpi.com/1422-0067/23/14/7663"
}