Publication detail

Hydrogel structure determined by fluorescent nanoparticles

MARKOVÁ, K. PEKAŘ, M.

Original Title

Type

abstract

Language

English

Original Abstract

This work focuses on the determining of the hydrogel structure using fluorescent nanoparticles. As the main method was chosen fluorescence lifetime imaging microscopy (FLIM) and subsequent analyzes were performed by specialized programs. One of these program is able to create basic FLIM image. The other program processes these images into Euclidean distance map. Thanks to them it is possible to determine the mesh size and show the approximate structure of the hydrogel network. It is also possible to create 3D structure of the hydrogel from FLIM images obtained from z-scan. The another type of measurement was using nanoparticles of different diameters and excitation and emmision wavelenghts. They make it possible to track sites of varying pore sizes within a single experiment.

Keywords

hydrogel, structure, agarose, fluorescence microscopy, FLIM

Authors

MARKOVÁ, K.; PEKAŘ, M.

Released

22. 10. 2021

Publisher

Tanger Ltd.

Location

Ostrava

ISBN

978-80-88365-00-6

Book

Nanocon 2021 – Abstracts

Pages count

BibTex

@misc{BUT179476,
  author="Kateřina {Marková} and Miloslav {Pekař}",
  title="Hydrogel structure determined by fluorescent nanoparticles",
  booktitle="Nanocon 2021 – Abstracts",
  year="2021",
  pages="1",
  publisher="Tanger Ltd.",
  address="Ostrava",
  isbn="978-80-88365-00-6",
  note="abstract"
}