Hemostatic antibacterial wound dressing for accelerated blood stop

abstract

English

INTRODUCTION: The bleeding is one of the most commonly occurring injuries. From minor scratches to lacerations and even combat wounds. These wounds could be very painful, be a place where infection enters a body and might lower a person's overall condition [1]. The answer to deal with these problems could be a new hemostatic antibacterial wound dressing that will accelerate hemostasis and have antibacterial properties. Our vision and the primary goal of this work was to develop, manufacture, and characterise the physical, chemical, and hemostatic properties in vitro of a hemostatic wound dressing from a mixture based on oxidised cellulose, chitosan, and carboxymethylcellulose. Using a combination of these materials should give us improved properties of the final product. Mixing substances in the right composition and ratios will allow us to take full advantage of the material properties synergy. METHODS: Dressing based on chitosan, oxidised cellulose and carboxymethylcellulose sodium salt were prepared in the form of sponges by the freeze-drying process. Prepared foams were characterised by infrared spectroscopy (ATR-FTIR) to confirm chemical composition. SEM images were obtained for morphology information (the porosity, size, and shape of pores). The absorption capacity of simulated fluids was determined according to ČSN EN 13726-1. For hemostatic properties, different in vitro methods were used, e.g., blood clotting assays, clotting time, and enzymatic test. RESULTS: The materials work as great hemostatic agents on their own, but when combined, the results for absorption capacity, clotting time and other results showed an interesting increase in desirable properties. DISCUSSION & CONCLUSIONS: The results showed a great synergy of used materials. The lyophilised sponges based on chitosan, carboxymethylcellulose and oxidised cellulose have very promising results for the absorption capacity and for the conducted in vitro hemostatic testing with porcine blood. These results show that using a mixture of commonly used hemostatic biomaterials could bring some improved results and the final product might have added value. ACKNOWLEDGEMENTS: This work was supported by the project Quality Internal Grants of BUT (KInG BUT), Reg. No. CZ.02.2.69 / 0.0 / 0.0 / 19_073 / 0016948, which is financed from the OP RDE. CzechNanoLab project LM2018110 funded by MEYS CR is gratefully acknowledged for the financial support of the measurements at CEITEC Nano Research Infrastructure. REFERENCES: [1] YANG, Yutong, Yongping LIANG, Jueying CHEN, Xianglong DUAN a GUO. Bioactive Materials. 2022, 8. ISSN 2452199X. doi:10.1016/j.bioactmat.2021.06.014

Hemostasis, wound dressing, blood stop, biopolymers

SEDLÁŘ, M.; KACVINSKÁ, K.; DVOŘÁK, R.; SOPUCH, T.; VOJTOVÁ, L.

13. 6. 2022

eCM (Eur Cell Mater / e Cells & Materials)

Jurmalla

P38

P38

44

https://www.ecmconferences.org/abstracts/2022/Collection2/c2.html