Publication detail

Degradation of P(3HB-co-4HB) Films in Simulated Body Fluids

VODIČKA, J. WIKARSKÁ, M. TRUDIČOVÁ, M. JUGLOVÁ, Z. POSPÍŠILOVÁ, A. KALINA, M. SLANINOVÁ, E. OBRUČA, S. SEDLÁČEK, P.

Original Title

Degradation of P(3HB-co-4HB) Films in Simulated Body Fluids

Type

journal article in Web of Science

Language

English

Original Abstract

A novel model of biodegradable PHA copolymer films preparation was applied to evaluate the biodegradability of various PHA copolymers and to discuss its biomedical applicability. In this study, we illustrate the potential biomaterial degradation rate affectability by manipulation of monomer composition via controlling the biosynthetic strategies. Within the experimental investigation, we have prepared two different copolymers of 3-hydroxybutyrate and 4-hydroxybutyrate-P(3HB-co-36 mol.% 4HB) and P(3HB-co-66 mol.% 4HB), by cultivating the thermophilic bacterial strain Aneurinibacillus sp. H1 and further investigated its degradability in simulated body fluids (SBFs). Both copolymers revealed faster weight reduction in synthetic gastric juice (SGJ) and artificial colonic fluid (ACF) than simple homopolymer P3HB. In addition, degradation mechanisms differed across tested polymers, according to SEM micrographs. While incubated in SGJ, samples were fragmented due to fast hydrolysis sourcing from substantially low pH, which suggest abiotic degradation as the major degradation mechanism. On the contrary, ACF incubation indicated obvious enzymatic hydrolysis. Further, no cytotoxicity of the waste fluids was observed on CaCO-2 cell line. Based on these results in combination with high production flexibility, we suggest P(3HB-co-4HB) copolymers produced by Aneurinibacillus sp. H1 as being very auspicious polymers for intestinal in vivo treatments.

Keywords

polyhydroxyalkanoates (PHA); Aneurinibacillus sp. H1; PHA copolymers; biodegradation; simulated body fluids

Authors

VODIČKA, J.; WIKARSKÁ, M.; TRUDIČOVÁ, M.; JUGLOVÁ, Z.; POSPÍŠILOVÁ, A.; KALINA, M.; SLANINOVÁ, E.; OBRUČA, S.; SEDLÁČEK, P.

Released

13. 5. 2022

Publisher

MDPI

Location

BASEL

ISBN

2073-4360

Periodical

Polymers

Year of study

14

Number

10

State

Swiss Confederation

Pages from

1

Pages to

14

Pages count

14

URL

https://www.mdpi.com/2073-4360/14/10/1990

Full text in the Digital Library

http://hdl.handle.net/11012/204434

BibTex

@article{BUT177660,
  author="Juraj {Vodička} and Monika {Wikarská} and Monika {Trudičová} and Zuzana {Juglová} and Aneta {Pospíšilová} and Michal {Kalina} and Eva {Slaninová} and Stanislav {Obruča} and Petr {Sedláček}",
  title="Degradation of P(3HB-co-4HB) Films in Simulated Body Fluids",
  journal="Polymers",
  year="2022",
  volume="14",
  number="10",
  pages="1--14",
  doi="10.3390/polym14101990",
  issn="2073-4360",
  url="https://www.mdpi.com/2073-4360/14/10/1990"
}