Publication detail

Conductive Polymer PEDOT:PSS-Based Platform for Embryonic Stem-Cell Differentiation

ŠAFAŘÍKOVÁ, E. EHLICH, J. STŘÍTESKÝ, S. VALA, M. WEITER, M. PACHERNÍK, J. KUBALA, L. VÍTEČEK, J.

Original Title

Type

journal article in Web of Science

Language

English

Original Abstract

Organic semiconductors are constantly gaining interest in regenerative medicine. Their tunable physico-chemical properties, including electrical conductivity, are very promising for the control of stem-cell differentiation. However, their use for combined material-based and electrical stimulation remains largely underexplored. Therefore, we carried out a study on whether a platform based on the conductive polymer poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS) can be beneficial to the differentiation of mouse embryonic stem cells (mESCs). The platform was prepared using the layout of a standard 24-well cell-culture plate. Polyethylene naphthalate foil served as the substrate for the preparation of interdigitated gold electrodes by physical vapor deposition. The PEDOT:PSS pattern was fabricated by precise screen printing over the gold electrodes. The PEDOT:PSS platform was able to produce higher electrical current with the pulsed-direct-current (DC) electrostimulation mode (1 Hz, 200 mV/mm, 100 ms pulse duration) compared to plain gold electrodes. There was a dominant capacitive component. In proof-of-concept experiments, mESCs were able to respond to such electrostimulation by membrane depolarization and elevation of cytosolic calcium. Further, the PEDOT:PSS platform was able to upregulate cardiomyogenesis and potentially inhibit early neurogenesis per se with minor contribution of electrostimulation. Hence, the present work highlights the large potential of PEDOT:PSS in regenerative medicine.

Keywords

conductive polymer; PEDOT; PSS; screen print; embryonic stem cells; electrostimulation

Authors

ŠAFAŘÍKOVÁ, E.; EHLICH, J.; STŘÍTESKÝ, S.; VALA, M.; WEITER, M.; PACHERNÍK, J.; KUBALA, L.; VÍTEČEK, J.

Released

20. 1. 2022

Publisher

MDPI

Location

BASEL

ISBN

1422-0067

Periodical

International Journal of Molecular Sciences

Year of study

Number

State

Swiss Confederation

Pages from

Pages to

Pages count

URL

https://www.mdpi.com/1422-0067/23/3/1107

Full text in the Digital Library

http://hdl.handle.net/11012/204601

BibTex

@article{BUT177314,
  author="Eva {Šafaříková} and Jiří {Ehlich} and Stanislav {Stříteský} and Martin {Vala} and Martin {Weiter} and Jiří {Pacherník} and Lukáš {Kubala} and Jan {Víteček}",
  title="Conductive Polymer PEDOT:PSS-Based Platform for Embryonic Stem-Cell Differentiation",
  journal="International Journal of Molecular Sciences",
  year="2022",
  volume="23",
  number="3",
  pages="1--16",
  doi="10.3390/ijms23031107",
  issn="1422-0067",
  url="https://www.mdpi.com/1422-0067/23/3/1107"
}