Publication detail

Assessment of Biotechnologically Important Filamentous Fungal Biomass by Fourier Transform Raman Spectroscopy

DZURENDOVÁ, S. SHAPAVAL, V. TAFINTSEVA, V. KOHLER, A. BYRTUSOVÁ, D. SZOTKOWSKI, M. MÁROVÁ, I. ZIMMERMANN, B.

Original Title

Assessment of Biotechnologically Important Filamentous Fungal Biomass by Fourier Transform Raman Spectroscopy

Type

journal article in Web of Science

Language

English

Original Abstract

Oleaginous filamentous fungi can accumulate large amount of cellular lipids and biopolymers and pigments and potentially serve as a major source of biochemicals for food, feed, chemical, pharmaceutical, and transport industries. We assessed suitability of Fourier transform (FT) Raman spectroscopy for screening and process monitoring of filamentous fungi in biotechnology. Six Mucoromycota strains were cultivated in microbioreactors under six growth conditions (three phosphate concentrations in the presence and absence of calcium). FT-Raman and FT-infrared (FTIR) spectroscopic data was assessed in respect to reference analyses of lipids, phosphorus, and carotenoids by using principal component analysis (PCA), multiblock or consensus PCA, partial least square regression (PLSR), and analysis of spectral variation due to different design factors by an ANOVA model. All main chemical biomass constituents were detected by FT-Raman spectroscopy, including lipids, proteins, cell wall carbohydrates, and polyphosphates, and carotenoids. FT-Raman spectra clearly show the effect of growth conditions on fungal biomass. PLSR models with high coefficients of determination (0.83-0.94) and low error (approximately 8%) for quantitative determination of total lipids, phosphates, and carotenoids were established. FT-Raman spectroscopy showed great potential for chemical analysis of biomass of oleaginous filamentous fungi. The study demonstrates that FT-Raman and FTIR spectroscopies provide complementary information on main fungal biomass constituents.

Keywords

oleaginous microorganisms; biodiesel; pigments; biopolymers; carotenoids; fatty acids; chitin; chitosan; fermentation; fungi

Authors

DZURENDOVÁ, S.; SHAPAVAL, V.; TAFINTSEVA, V.; KOHLER, A.; BYRTUSOVÁ, D.; SZOTKOWSKI, M.; MÁROVÁ, I.; ZIMMERMANN, B.

Released

23. 6. 2021

Publisher

MDPI

Location

BASEL

ISBN

2076-2607

Periodical

Microorganisms

Year of study

22

Number

13

State

Swiss Confederation

Pages from

1

Pages to

23

Pages count

23

URL

https://www.mdpi.com/1422-0067/22/13/6710

Full text in the Digital Library

http://hdl.handle.net/11012/204970

BibTex

@article{BUT177294,
  author="Simona {Dzurendová} and Volha {Shapaval} and Valeria {Tafintseva} and Achim {Kohler} and Dana {Byrtusová} and Martin {Szotkowski} and Ivana {Márová} and Boris {Zimmermann}",
  title="Assessment of Biotechnologically Important Filamentous Fungal Biomass by Fourier Transform Raman Spectroscopy",
  journal="Microorganisms",
  year="2021",
  volume="22",
  number="13",
  pages="1--23",
  doi="10.3390/ijms22136710",
  issn="2076-2607",
  url="https://www.mdpi.com/1422-0067/22/13/6710"
}