Publication detail

Complete Genome Sequence of the Type Strain Tepidimonas taiwanensis LMG 22826T, a Thermophilic Alkaline Protease and Polyhydroxyalkanoate Producer

HEŘMÁNKOVÁ, K. KOUŘILOVÁ, X. PERNICOVÁ, I. BEZDÍČEK, M. LENGEROVÁ, M. OBRUČA, S. SEDLÁŘ, K.

Original Title

Complete Genome Sequence of the Type Strain Tepidimonas taiwanensis LMG 22826T, a Thermophilic Alkaline Protease and Polyhydroxyalkanoate Producer

Type

journal article in Web of Science

Language

English

Original Abstract

Tepidimonas taiwanensis is a moderately thermophilic, Gram-negative, rod-shaped, chemoorganoheterotrophic, motile bacterium. The alkaline protease producing type strain Tepidimonas taiwanensis LMG 22826T was recently reported to also be a promising producer of polyhydroxyalkanoates (PHAs) – renewable and biodegradable polymers representing an alternative to conventional plastics. Here, we present its first complete genome sequence which is also the first complete genome sequence of the whole species. The genome consists of a single 2,915,587 bp long circular chromosome with GC content of 68.75%. Genome annotation identified 2,764 genes in total while 2,634 open reading frames belonged to protein-coding genes. Although functional annotation of the genome and division of genes into Clusters of Orthologous Groups (COGs) revealed a relatively high number of 694 genes with unknown function or unknown COG, the majority of genes were assigned a function. Most of the genes, 406 in total, were involved in energy production and conversion, and amino acid transport and metabolism. Moreover, particular key genes involved in the metabolism of PHA were identified. Knowledge of the genome in connection with the recently reported ability to produce bioplastics from the waste stream of wine production makes T. taiwanensis LMG 22826T an ideal candidate for further genome engineering as a bacterium with high biotechnological potential.

Keywords

Tepidimonas taiwanensis; polyhydroxyalkanoates; hybrid assembly; Oxford Nanopore Technologies; functional annotation; alkaline protease

Authors

HEŘMÁNKOVÁ, K.; KOUŘILOVÁ, X.; PERNICOVÁ, I.; BEZDÍČEK, M.; LENGEROVÁ, M.; OBRUČA, S.; SEDLÁŘ, K.

Released

15. 12. 2021

Publisher

Oxford University Press

ISBN

1759-6653

Periodical

GENOME BIOL EVOL

Year of study

13

Number

12

State

United Kingdom of Great Britain and Northern Ireland

Pages from

1

Pages to

5

Pages count

5

URL

https://academic.oup.com/gbe/advance-article/doi/10.1093/gbe/evab280/6462190

Full text in the Digital Library

http://hdl.handle.net/11012/203302

BibTex

@article{BUT175376,
  author="Kristýna {Heřmánková} and Xenie {Kouřilová} and Iva {Buchtíková} and Matěj {Bezdíček} and Martina {Lengerová} and Stanislav {Obruča} and Karel {Sedlář}",
  title="Complete Genome Sequence of the Type Strain Tepidimonas taiwanensis LMG 22826T, a Thermophilic Alkaline Protease and Polyhydroxyalkanoate Producer",
  journal="GENOME BIOL EVOL",
  year="2021",
  volume="13",
  number="12",
  pages="1--5",
  doi="10.1093/gbe/evab280",
  issn="1759-6653",
  url="https://academic.oup.com/gbe/advance-article/doi/10.1093/gbe/evab280/6462190"
}