Instrumental characterization of Cyanobacteria as Polyhydroxybutyrate Producer

abstract

English

In this study, two cyanobacterial cultures from genera Synechocystis were used, namely Synechocystic sp. PCC 6803 and Synechocystis There were optimized and used several various approaches including microscopic techniques such as Cryo-scanning electron microscopy (Cryo-SEM) and transmission electron microscopy (TEM) and fluorescence lifetime imaging microscopy using Nile red as fluorescent probe (FLIM). Due to these instrumental techniques, morphology of intracellular space and surface of cells were characterized. Next group of methods which were employed were spectroscopic techniques such as UV-Vis spectroscopy measured in two modes (turbidimetry and integrating sphere) and Fourier transform infrared spectroscopy (FTIR). All these diverse techniques were used for detection and characterization of pigments (chlorophylls, carotenoids, phycocyanin etc.) and PHAs, in our case poly(3-hydroxybutyrate) (P3HB). To verify results, gas chromatography (GC) was employed, concretely for determination of amount of P3HB in biomass. Cyanobacteria was also characterized as polyhydroxybutyrate producer by flow cytometer which could count cells and at the same time distinguish cells including P3HB and without P3HB due to fluorescent probe called BODIPY and live/dead fluorescent probe SYTO Blue. Based on the results, P3HB content in cyanobacteria cells was determined and also overall fitness of the cells.

Cyanobacteria, fluorescent probe, microscopic techniques, poly(3-hydroxybutyrate), spectroscopy, chromatography

SLANINOVÁ, E.; ČERNAYOVÁ, D.; ŠEDRLOVÁ, Z.; MRÁZOVÁ, K.; SEDLÁČEK, P.; NEBESÁŘOVÁ, J.; OBRUČA, S.

29. 9. 2021

1307-6892

World Academy of Science, Engineering and Technology

Republic of Turkey

75

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