Publication detail

Light Scattering Techniques as a Key for Accessing of the Molecular Weight and Size of Humic Substances Originating from Different Source Materials

KALINA, M. ŠIRŮČEK, D. SOVOVÁ, Š. ENEV, V.

Original Title

Type

abstract

Language

English

Original Abstract

Abstract. This contribution is focused on the utilization of light scattering techniques (dynamic, static and electrophoretic) for purposes of an in-depth study on the aggregation, conformation changes and colloidal stability of soluble fractions of humic substances in aqueous solutions. For these purposes, the behaviour of various soluble humic fractions (mainly humic and fulvic) was studied and compared. The heterogeneity of humic molecules in their aqueous solutions was firstly necessary to decrease before their subsequent instrumental light scattering characterization. For these purposes, the methods of size exclusion chromatography and asymmetric flow-field flow fractionation were used. The obtained results showed similar basic structural features in the case of the individual humic acids isolated from different source materials as well as between the various studied fulvic acids. Different aggregation behaviour was observed in the case of the commertial lignohumate sample.

Keywords

humic substances, asymmetric flow-field flow fractionation, light scattering techniques, molecular weight, molecular size, polydispersity, size exclusion chromatography

Authors

KALINA, M.; ŠIRŮČEK, D.; SOVOVÁ, Š.; ENEV, V.

Released

15. 8. 2021

Pages from

Pages to

Pages count

BibTex

@misc{BUT172715,
  author="Michal {Kalina} and David {Širůček} and Šárka {Bačovská} and Vojtěch {Enev}",
  title="Light Scattering Techniques as a Key for Accessing of the Molecular Weight and Size of Humic Substances Originating from Different Source Materials",
  year="2021",
  pages="1--2",
  note="abstract"
}