Publication detail

Lyophilized Polyvinylpyrrolidone Hydrogel for Culture of Human Oral Mucosa Stem Cells

OLIVER, C. IBAÑEZ, R. FLORES-MERINO, M. VOJTOVÁ, L. ŠALPLACHTA, J. ČELKO, L. KAISER, J. MONTUFAR JIMENEZ, E.

Original Title

Type

journal article in Web of Science

Language

English

Original Abstract

This work shows the synthesis of a polyvinylpyrrolidone (PVP) hydrogel by heat-activated polymerization and explores the production of hydrogels with an open porous network by lyophilisation to allow the three-dimensional culture of human oral mucosa stem cells (hOMSCs). The swollen hydrogel showed a storage modulus similar to oral mucosa and elastic solid rheological behaviour without sol transition. A comprehensive characterization of porosity by scanning electron microscopy, mercury intrusion porosimetry and nano-computed tomography (with spatial resolution below 1 mu m) showed that lyophilisation resulted in the heterogeneous incorporation of closed oval-like pores in the hydrogel with broad size distribution (5 to 180 mu m, d(50) = 65 mu m). Human oral mucosa biopsies were used to isolate hOMSCs, expressing typical markers of mesenchymal stem cells in more than 95% of the cell population. Direct contact cytotoxicity assay demonstrated that PVP hydrogel have no negative effect on cell metabolic activity, allowing the culture of hOMSCs with normal fusiform morphology. Pore connectivity should be improved in future to allow cell growth in the bulk of the PVP hydrogel.

Keywords

polyvinylpyrrolidone; hydrogel; lyophilisation; nano-computed tomography; porosity; oral mucosa; stem cell

Authors

OLIVER, C.; IBAÑEZ, R.; FLORES-MERINO, M.; VOJTOVÁ, L.; ŠALPLACHTA, J.; ČELKO, L.; KAISER, J.; MONTUFAR JIMENEZ, E.

Released

5. 1. 2021

Publisher

MDPI

Location

BASEL

ISBN

1996-1944

Periodical

Materials

Year of study

Number

State

Swiss Confederation

Pages from

Pages to

Pages count

URL

https://www.mdpi.com/1996-1944/14/1/227

Full text in the Digital Library

http://hdl.handle.net/11012/195922

BibTex

@article{BUT168361,
  author="Carolina {Oliver} and Raúl Rosales {Ibañez} and Miriam {Flores-Merino} and Lucy {Vojtová} and Jakub {Šalplachta} and Ladislav {Čelko} and Jozef {Kaiser} and Edgar Benjamin {Montufar Jimenez}",
  title="Lyophilized Polyvinylpyrrolidone Hydrogel for Culture of Human Oral Mucosa Stem Cells",
  journal="Materials",
  year="2021",
  volume="14",
  number="1",
  pages="1--14",
  doi="10.3390/ma14010227",
  issn="1996-1944",
  url="https://www.mdpi.com/1996-1944/14/1/227"
}