Publication detail

Preparation and characterisation of organic UV filters based on combined PHB/liposomes with natural phenolic compounds

PAVELKOVÁ, R. MATOUŠKOVÁ, P. HOOVÁ, J. POŘÍZKA, J. MÁROVÁ, I.

Original Title

Preparation and characterisation of organic UV filters based on combined PHB/liposomes with natural phenolic compounds

Type

journal article in Web of Science

Language

English

Original Abstract

In this study preparation and characterization of new UV-protecting systems based on liposomes/polyhydroxybutyrate (PHB) with encapsulated coffee extracts are presented. Green and roasted coffee extracts with high phenolics content, high antioxidant activity and sun protection factor (SPF) value 40–50 were used as model organic UV filters and encapsulated into liposomes and PHB-liposomes. Particle size and colloid stability was observed by dynamic light scattering and zeta-potential. Toxicity of particles was tested by MTT and LDH assay on HaCaT cell line. All prepared samples showed moderate or high encapsulation efficiency. Addition of PHB up to 50 % of lecithin led to increased size and stability. As optimal addition of 20 % PHB into liposome particles was found leading to optimum size and processing of particles, to high UV-protective effect as well as to increased colloid stability and SPF value during long-term storage. Significant differences in cell viability were found in cytotoxicity studies after exposure of keratinocytes to liposomes with different PHB content. Newly fabricated PHB-liposome particles with coffee extract were not found as toxic for HaCaT cells and in LDH test up to 12 %. These particles can act as active carriers for organic sunscreen components in combination with UV-protective effect of PHB.

Keywords

Nanoparticles, PHB-liposomes, Stability, Cytotoxicity, UV protection, Coffea arabica

Authors

PAVELKOVÁ, R.; MATOUŠKOVÁ, P.; HOOVÁ, J.; POŘÍZKA, J.; MÁROVÁ, I.

Released

1. 10. 2020

Publisher

Elsevier

ISBN

0168-1656

Periodical

Journal of Biotechnology

Year of study

324

Number

1

State

Kingdom of the Netherlands

Pages from

1

Pages to

10

Pages count

10

URL

https://www.sciencedirect.com/science/article/pii/S2590155920300081

Full text in the Digital Library

http://hdl.handle.net/11012/195851

BibTex

@article{BUT166337,
  author="Renata {Uhlířová} and Petra {Skoumalová} and Julie {Hoová} and Jaromír {Pořízka} and Ivana {Márová}",
  title="Preparation and characterisation of organic UV filters based on combined PHB/liposomes with natural phenolic compounds",
  journal="Journal of Biotechnology",
  year="2020",
  volume="324",
  number="1",
  pages="1--10",
  doi="10.1016/j.btecx.2020.100021",
  issn="0168-1656",
  url="https://www.sciencedirect.com/science/article/pii/S2590155920300081"
}