Publication detail

Introducing the Newly Isolated BacteriumAneurinibacillussp. H1 as an Auspicious Thermophilic Producer of Various Polyhydroxyalkanoates (PHA) Copolymers-2. Material Study on the Produced Copolymers

SEDLÁČEK, P. PERNICOVÁ, I. NOVÁČKOVÁ, I. KOUŘILOVÁ, X. KALINA, M. KOVALČÍK, A. KOLLER, M. NEBESAROVA, J. KRZYZANEK, V. HRUBANOVA, K. MÁSILKO, J. SLANINOVÁ, E. TRUDIČOVÁ, M. OBRUČA, S.

Original Title

Introducing the Newly Isolated BacteriumAneurinibacillussp. H1 as an Auspicious Thermophilic Producer of Various Polyhydroxyalkanoates (PHA) Copolymers-2. Material Study on the Produced Copolymers

Type

journal article in Web of Science

Language

English

Original Abstract

Aneurinibacillussp. H1 is a promising, moderately thermophilic, novel Gram-positive bacterium capable of the biosynthesis of polyhydroxyalkanoates (PHA) with tunable monomer composition. In particular, the strain is able to synthesize copolymers of 3-hydroxybutyrate (3HB), 4-hydroxybutyrate (4HB) and 3-hydroxyvalerate (3HV) with remarkably high 4HB and 3HV fractions. In this study we performed an in-depth material analysis of PHA polymers produced byAneurinibacillussp. H1 in order to describe how the monomer composition affects fundamental structural and physicochemical parameters of the materials in the form of solvent-casted films. Results of infrared spectroscopy, X-ray diffractometry and thermal analysis clearly show that controlling the monomer composition enables optimization of PHA crystallinity both qualitatively (the type of the crystalline lattice) and quantitatively (the overall degree of crystallinity). Furthermore, resistance of the films against thermal and/or enzymatic degradation can also be manipulated by the monomer composition. Results of this study hence confirmAneurinibacillussp. H1 as an auspicious candidate for thermophilic production of PHA polymers with material properties that can be tuned together with their chemical composition by the corresponding adjustment of the cultivation process.

Keywords

polyhydroxyalkanoates; thermophiles; Aneurinibacillussp; P(3HB-co-4HB); P(3HB-co-3HV-co-4HB); crystallinity

Authors

SEDLÁČEK, P.; PERNICOVÁ, I.; NOVÁČKOVÁ, I.; KOUŘILOVÁ, X.; KALINA, M.; KOVALČÍK, A.; KOLLER, M.; NEBESAROVA, J.; KRZYZANEK, V.; HRUBANOVA, K.; MÁSILKO, J.; SLANINOVÁ, E.; TRUDIČOVÁ, M.; OBRUČA, S.

Released

5. 6. 2020

Publisher

MDPI

Location

Switzerland

ISBN

2073-4360

Periodical

Polymers

Year of study

12

Number

6

State

Swiss Confederation

Pages from

1

Pages to

18

Pages count

18

URL

https://www.mdpi.com/2073-4360/12/6/1298

Full text in the Digital Library

http://hdl.handle.net/11012/194618

BibTex

@article{BUT164653,
  author="Petr {Sedláček} and Iva {Buchtíková} and Ivana {Nováčková} and Xenie {Kouřilová} and Michal {Kalina} and Adriána {Kovalčík} and Martin {Koller} and Jana {Nebesářová} and Vladislav {Krzyžánek} and Kamila {Hrubanová} and Jiří {Másilko} and Eva {Slaninová} and Monika {Trudičová} and Stanislav {Obruča}",
  title="Introducing the Newly Isolated BacteriumAneurinibacillussp. H1 as an Auspicious Thermophilic Producer of Various Polyhydroxyalkanoates (PHA) Copolymers-2. Material Study on the Produced Copolymers",
  journal="Polymers",
  year="2020",
  volume="12",
  number="6",
  pages="1--18",
  doi="10.3390/polym12061298",
  issn="2073-4360",
  url="https://www.mdpi.com/2073-4360/12/6/1298"
}