Publication detail

Semi-quantitative estimation of soya protein-based additives in meat products using real-time polymerase chain reaction

ŠMÍD, J. GODALOVA, Z. PIKNOVA, L. SIEKEL, P. KUCHTA, T.

Original Title

Semi-quantitative estimation of soya protein-based additives in meat products using real-time polymerase chain reaction

Type

journal article in Web of Science

Language

English

Original Abstract

A real-time polymerase chain reaction (PCR) detection system with a TaqMan probe for quantification of soya in meat products was developed and evaluated. The detection system targeted the Le1 gene encoding for soya lectin and exhibited 100% inclusivity (9 Glycine max cultivars) and 100% exclusivity (22 plant samples) for the detection of soya. Analytical sensitivity of the assay was 5.75 pg DNA per reaction, which corresponds to approx. 2 haploid genome equivalents. Practical detection and quantification limits for three different soya protein-based additives (protein concentrate, protein isolate, okara), in liver pate as a matrix, were at a level of 0.1 g.kg(-1). The calibration lines for each series of model samples containing individual soya protein-based additives were statistically identical in the range from limit of quantification to 100 g.kg(-1), which means that the same calibration line could be used for all soya protein-based additives. The developed real-time PCR facilitated semi-quantitative estimation of soya protein-based additives in meat products, which may be useful for the control of labelling of meat products.

Keywords

soya; protein; meat; polymerase chain reaction

Authors

ŠMÍD, J.; GODALOVA, Z.; PIKNOVA, L.; SIEKEL, P.; KUCHTA, T.

Released

1. 4. 2015

Publisher

VUP FOOD RESEARCH INST, BRATISLAVA

ISBN

1336-8672

Periodical

Journal of Food and Nutrition Research

Year of study

54

Number

2

State

Slovak Republic

Pages from

165

Pages to

170

Pages count

6

URL

http://agris.fao.org/agris-search/search.do?recordID=SK2016013970

BibTex

@article{BUT163481,
  author="ŠMÍD, J. and GODALOVA, Z. and PIKNOVA, L. and SIEKEL, P. and KUCHTA, T.",
  title="Semi-quantitative estimation of soya protein-based additives in meat products using real-time polymerase chain reaction",
  journal="Journal of Food and Nutrition Research",
  year="2015",
  volume="54",
  number="2",
  pages="165--170",
  issn="1336-8672",
  url="http://agris.fao.org/agris-search/search.do?recordID=SK2016013970"
}