Application of evolutionary engineering for enhancement of robustness and producing capacity of selected microbial PHA producers

abstract

English

Selected PHAs producing microorganisms Cupriavidus necator H16 (CCM 3726) and Halomonas halophila (CCM 3662) have been exposed to several biotechnologically relevant stressors. Whereas C. necator H16 was exposed to osmotic stress (NaCl) and presence of copper representing anthropogenic pollutant, H. halophila was exposed to acetic and levulinic acid; both of these are components of hydrolysates of lignocellulosic biomass. Experiments were provided using multiple serial transfers of cultures in Erlenmeyer flasks after 48 hours of cultivation. Basic screening of every step included determination of optical density of cultures, determination of dry cell weight gravimetrically and GC-FID analysis of PHAs. Potential of PHAs accumulation, testing of robustness against various stress factors, changes in genome, Raman spectra comparison etc. were determined within comparison of selected evolved strains with wild-type ones.

evolutionary engineering, polyhydroxyalkanoates, C. necator H16, H. halophila

NOVÁČKOVÁ, I.; KUČERA, D.; POŘÍZKA, J.; PERNICOVÁ, I.; SEDLÁČEK, P.; OBRUČA, S.

30. 9. 2019

978-80-210-9373-7

The Biomania student scientific meeting and Eusynbios symposium 2019 - Book of Abstracts

1

120

120

140