Publication detail

Pre-treatment and elution of amino acids from dried blood spots for direct determination by capillary electrophoresis.

RYŠAVÁ, L. DVOŘÁK, M. MIKOVÁ, B. KUBÁŇ, P.

Original Title

Pre-treatment and elution of amino acids from dried blood spots for direct determination by capillary electrophoresis.

Type

presentation, poster

Language

English

Original Abstract

In this contribution, the process of sampling the DBSs and their subsequent elution with deionized water, methanol, acetonitrile, and optionally with mixtures thereof is shown. Various effects of the co-eluted matrix components on CE-C4D were observed for the selected elution solutions and are comprehensively described. The CE-C4D method was optimized for baseline separation of biogenic amino acids and indicated excellent analytical parameters and high stability of the separation system. CE-C4D was used for the direct analysis of the DBS eluates from healthy donors with amino acid concentrations at physiological levels. Finally, the method was applied to the determination of selected amino acids from DBS samples, which are related to common inborn metabolic disorders, such as maple syrup urine disease and phenylketonuria.

Keywords

capillary electrophoresis, amino acid, dry blood spots

Authors

RYŠAVÁ, L.; DVOŘÁK, M.; MIKOVÁ, B.; KUBÁŇ, P.

Released

3. 10. 2019

Pages from

20

Pages to

20

Pages count

1

URL

https://asep.lib.cas.cz/arl-cav/cs/csg/?repo=crepo1&key=12859950138

BibTex

@misc{BUT159067,
  author="Lenka {Ryšavá} and Miloš {Dvořák} and Miková {Blanka} and Pavel {Kubáň}",
  title="Pre-treatment and elution of amino acids from dried blood spots for direct determination by capillary electrophoresis.",
  year="2019",
  pages="20--20",
  url="https://asep.lib.cas.cz/arl-cav/cs/csg/?repo=crepo1&key=12859950138",
  note="presentation, poster"
}