Publication detail

A simple and efficient procedure for capillary blood spiking with basic and acidic drugs for subsequent dried blood spot analysis.

DVOŘÁK, M. RYŠAVÁ, L. KUBÁŇ, P.

Original Title

A simple and efficient procedure for capillary blood spiking with basic and acidic drugs for subsequent dried blood spot analysis.

Type

presentation, poster

Language

English

Original Abstract

In the actual contribution, a simple process was developed and optimized for effective IS addition and DBS elution. A group of acidic and/or basic drugs, with different chemical characteristics, was added to the collected capillary blood in different ways and the mixture was transferred onto the DBS collection card (903®Whatman™). Various elution procedures were also examined and are discussed. Direct injection of the eluted DBS sample into CE-UV ensured a rapid and reliable determination of the drugs without the need for any additional pretreatment steps (extraction, preconcentration). The contents of analytes in the dissolved eluates were determined and recoveries >70% were achieved for the optimized elution systems with satisfactory repeatability (RSD <5%).

Keywords

capillary electrophoresis, amino acid,dry blood spots

Authors

DVOŘÁK, M.; RYŠAVÁ, L.; KUBÁŇ, P.

Released

3. 10. 2019

Pages from

25

Pages to

25

URL

https://asep.lib.cas.cz/arl-cav/cs/csg/?repo=crepo1&key=60490652627

BibTex

@misc{BUT159066,
  author="DVOŘÁK, M. and RYŠAVÁ, L. and KUBÁŇ, P.",
  title="A simple and efficient procedure for capillary blood spiking with basic and acidic drugs for subsequent dried blood spot analysis.",
  year="2019",
  pages="25--25",
  url="https://asep.lib.cas.cz/arl-cav/cs/csg/?repo=crepo1&key=60490652627",
  note="presentation, poster"
}