Publication detail

An appropriate method for assessing hydrogel pore sizes by cryo-SEM

ADÁMKOVÁ, K. TRUDIČOVÁ, M. SEDLÁČEK, P. KRZYŽÁNEK, V. HRUBANOVÁ, K.

Original Title

Type

conference paper

Language

English

Original Abstract

The aim of our work was to examine and describe ultrastructure of the agarose hydrogel and any possible structural concentration dependencies, and to assess the distribution and size of pores of agarose hydrogel in dependence on its concentration. Four concentrations were prepared (0.5 %, 1.0 %, 2.0 % and 4.0 % of dry weight content) and cryo-SEM and turbidimetry methods were executed on wet (original) samples in order to image the ultrastructure and measure the pore sizes within. Reasonable results were obtained for the wet samples as they were closer to their native state they are usually used for applications in. Cryo-SEM and turbidimetry provided comparable results of pore diameters and allowed to compare pore diameters dependant on the concentrations; moreover, it showed more detailed and realistic structure.

Keywords

hydrogel, cryo-SEM, freezing methods, agarose, image analysis

Authors

ADÁMKOVÁ, K.; TRUDIČOVÁ, M.; SEDLÁČEK, P.; KRZYŽÁNEK, V.; HRUBANOVÁ, K.

Released

1. 1. 2019

Publisher

TANGER Ltd.

Location

Ostrava

ISBN

9788087294895

Book

NANOCON 2018 - Conference Proceedings, 10th Anniversary International Conference on Nanomaterials - Research and Application

Edition

Edition number

Pages from

415

Pages to

420

Pages count

BibTex

@inproceedings{BUT157932,
  author="ADÁMKOVÁ, K. and TRUDIČOVÁ, M. and SEDLÁČEK, P. and KRZYŽÁNEK, V. and HRUBANOVÁ, K.",
  title="An appropriate method for assessing hydrogel pore sizes by cryo-SEM",
  booktitle="NANOCON 2018 - Conference Proceedings, 10th Anniversary International Conference on Nanomaterials - Research and Application",
  year="2019",
  series="1",
  number="1",
  pages="415--420",
  publisher="TANGER Ltd.",
  address="Ostrava",
  isbn="9788087294895"
}