Publication detail

Bioelectronical device for electrostimulation of stem cells

Jiří Ehlich, Ota Salyk, Stanislav Stříteský, Lukáš Omasta, Eva Šafaříková, Jan Víteček, Lukáš Kubala, Martin Vala, Martin Weiter

Original Title

Type

abstract

Language

English

Original Abstract

Human induced pluripotent stem cells (iPSCs) show the unique ability of differentiation to any cell type within an organism (e.g. neurons, myocytes, bone cells, etc.). Thus they may serve as an excellent tool in regenerative medicine. However the major challenge is to make them differentiate into particular cell line and even then their immature phenotypes limit their therapeutic application in clinical practice. Current practice capitalizes on mechanical and chemical cues, such as special scaffolds and biochemical factors to facilitate differentiation [1-3]. Recently it has been shown that electric stimulation may promote stem cell differentiation into electrogenic cell types such as neurons and cardiomyocytes [1-3]. However the inorganic materials known for not ideal biocompatibility were used as electrodes for electrostimulation and there was no clear clue between the electrostimulation pattern and the outcome of differentiation [1-3]. Our work tries to determine whether electrical stimulation of various voltage, current and frequency in combination with various organic semiconductors used as electrodes could influence the differentiation and maturation of mouse induced pluripotent stem cells to other types of cells. The prototype biolectronical device for electrostimulation is be presented. The device is initial platform for testing various organic semiconductors based on poly(3,4-ethylenedioxythiophene) (PEDOT) with various counter anions such as polystyrene sulfonate (PSS), hyaluronic acid (HA) etc. with chemically modified surfaces in respect to biocompatibility [4]. Device is able to apply electric fields to tested iPSCs in various modes. Premature results of baseline iPSCs electric stimulation will be also presented.

Keywords

Bioelectronics, electrostimulation, stem cells, PEDOT:PSS, organic semiconductors

Authors

Jiří Ehlich, Ota Salyk, Stanislav Stříteský, Lukáš Omasta, Eva Šafaříková, Jan Víteček, Lukáš Kubala, Martin Vala, Martin Weiter

Released

12. 9. 2018

Publisher

Vysoké učení technické v Brně, Fakulta chemická, Purkyňova 464/118, 612 00 Brno

Location

Brno

ISBN

978-80-214-5488-0

Book

7th Meeting on Chemistry and Life 2018. Book of abstracts

Pages count

BibTex

@misc{BUT155825,
  author="Jiří {Ehlich} and Ota {Salyk} and Lukáš {Omasta} and Stanislav {Stříteský} and Martin {Vala} and Martin {Weiter} and Jan {Víteček} and Lukáš {Kubala}",
  title="Bioelectronical device for electrostimulation of stem cells",
  booktitle="7th Meeting on Chemistry and Life 2018. Book of abstracts",
  year="2018",
  pages="1",
  publisher="Vysoké učení technické v Brně, Fakulta chemická, Purkyňova 464/118, 612 00 Brno",
  address="Brno",
  isbn="978-80-214-5488-0",
  note="abstract"
}