Antimicrobial activity of polyelectrolyte-surfactant hydrogels

abstract

English

Polyelectrolytes can interact with oppositely charged surfactants and under special conditions can form hydrogels. These hydrogels contain hydrophobic cores which are form from micelle-like nanostructures and due to these hydrophobic core, hydrogels are able to solubilise hydrophobic substances (such as fluorescent probes, drugs) and due to this fact hydrogels can be interesting point of interest for medical applications. Hydrogels combine the behavior of liquids and solids in a soft matter and their final behavior depends on the density of its network or the density of crosslinks connecting the network [1]. Network density and porous microstructure of hydrogels are very important and also affects the diffusion characteristics of hydrogels. This research was focused on testing the antimicrobial activity of the hydrogels which is based on diffusion methods. For this study were used hydrogels which were prepared from cationized dextran (DEAE) and negatively charged surfactant sodium dodecyl sulphate (SDS) and next type of hydrogels were hydrogels prepared from hyaluronic acid and positively charged surfactant carbethopendecinium bromide (Septonex). For determination of antimicrobial effect of prepared hydrogels agar disc/well diffusion method and broth dilution method (which is more sensitive) were used. Antimicrobial activity was tested against a gram-negative bacterium Serratia marcescens (SM), gram-positive bacterium Micrococcus luteus (ML) and against yeast Candida glabrata (CG) too. Antimicrobial tests were focused on testing the antimicrobial activity of the hydogels after preparation and subsequently after the washing process. Supernatants were also subjected to testing. All hydrogels showed antimicrobial effect against all of microbial strains after preparation. After the washing process antimicrobial activity in dextran hydrogels was weaker. Supernatants from dextrane hydrogels showed no antimicrobial activity against all of microbial strains but in the other side supernatants from hyaluronic hydrogels showed antimicrobial effect against gram-positive and gram-negative bacterial strains only and against yeast they showed no antimicrobial activity too.

hydrogels, polyelectrolyte, surfactant, antimicrobial activity

MATOUŠKOVÁ, P.; MRAVEC, F.; VELCER, T.; PEKAŘ, M.; JARÁBKOVÁ, S.

2. 9. 2018

University of Ljubljana, Faculty of Chemistry and Chemical Technology, Večna pot 113, 1000 Ljubljana, Slovenia

Ljubljana, Slovenia

978-961-6756-90-7

Book of Abstracts of The 32nd Conference of the European Colloid and Interface Society - ECIS2018

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