Publication detail

A quantitative analysis of 3D-cell distribution in regenerating muscle-skeletal system with synchrotron X-ray computed microtomography

TESAŘOVÁ M., MANCINI L., SIMON A., ADAMEYKO I., KAUCKÁ M., ELEWA A., LANZAFAME G., ZHANG Y., KALASOVÁ D., SZAROWSKÁ B., ZIKMUND T., NOVOTNÁ M., KAISER J.

Original Title

A quantitative analysis of 3D-cell distribution in regenerating muscle-skeletal system with synchrotron X-ray computed microtomography

Type

journal article in Web of Science

Language

English

Original Abstract

One of the greatest enigmas of modern biology is how the geometry of muscular and skeletal structures are created and how their development is controlled during growth and regeneration. Scaling and shaping of vertebrate muscles and skeletal elements has always been enigmatic and required an advanced technical level in order to analyse the cell distribution in 3D. In this work, synchrotron X-ray computed microtomography (μCT) and chemical contrasting has been exploited for a quantitative analysis of the 3D-cell distribution in tissues of a developing salamander (Pleurodeles waltl) limb – a key model organism for vertebrate regeneration studies. We mapped the limb muscles, their size and shape as well as the number and density of cells within the extracellular matrix of the developing cartilage. By using tomographic approach, we explored the polarity of the cells in 3D, in relation to the structure of developing joints. We found that the polarity of chondrocytes correlates with the planes in joint surfaces and also changes along the length of the cartilaginous elements. Our approach generates data for the precise computer simulations of muscle-skeletal regeneration using cell dynamics models, which is necessary for the understanding how anisotropic growth results in the precise shapes of skeletal structures.

Keywords

CT, tomography, 3D, cell, salamander

Authors

TESAŘOVÁ M., MANCINI L., SIMON A., ADAMEYKO I., KAUCKÁ M., ELEWA A., LANZAFAME G., ZHANG Y., KALASOVÁ D., SZAROWSKÁ B., ZIKMUND T., NOVOTNÁ M., KAISER J.

Released

20. 9. 2018

Publisher

Springer Nature

ISBN

2045-2322

Periodical

Scientific Reports

Year of study

8

Number

1

State

United Kingdom of Great Britain and Northern Ireland

Pages from

1

Pages to

13

Pages count

12

URL

http://link.springer.com/article/10.1038/s41598-018-32459-2

Full text in the Digital Library

http://hdl.handle.net/11012/85329

BibTex

@article{BUT150099,
  author="Markéta {Kaiser} and Dominika {Kalasová} and Tomáš {Zikmund} and Marie {Novotná} and Jozef {Kaiser}",
  title="A quantitative analysis of 3D-cell distribution in regenerating muscle-skeletal system with synchrotron X-ray computed microtomography",
  journal="Scientific Reports",
  year="2018",
  volume="8",
  number="1",
  pages="1--13",
  doi="10.1038/s41598-018-32459-2",
  issn="2045-2322",
  url="http://link.springer.com/article/10.1038/s41598-018-32459-2"
}