Publication detail

BIOCONVERSION OF LOW-COST FAT MATERIALS INTO HIGH-VALUE PUFA-CAROTENOID-RICH BIOMASS

M. Vysoká, M. Szotkowski, K. Bradáčová, J. Holub, P. Matoušková, S. Šimanský, M. Čertík, I. Márová

Original Title

Type

presentation, poster

Language

English

Original Abstract

Carotenoids are natural pigments occurring in plants and many microorganisms, such as algae, yeast and bacteria. Carotenoid yeasts are organisms capable of producing large amounts of biomass, enriched with mono and polyunsaturated fatty acids, with the use of lowcost waste substrates. Due to their antioxidant properties or antitumor and cancer-preventative action in cell lines properties, carotenoids find use in the food, agricultural or pharmaceutical industries. Lipids are used as biofuels and polyunsaturated fatty acids have a positive effect on human health, saturated fatty acids are therefore primarily a source of energy. Carotenoids, coenzyme Q and ergosterol in cells were determined by HPLC/PDA/MS. The lipid content and fatty acid profiles were analyzed by gas chromatography (GC/FID). Yeasts were cultivated on media with different carbon sources – glucose, glycerol and waste fat material from livestock production. For yeast cultivation, fat was used in its pure form or after basic, acidic or enzymatic hydrolysis. In addition, the use of various emulsifiers (Tween, PEG 400, Fatty acid salts) was tested. For these experiments Cystofilobasidium, Sporobolomycetes and Rhodotorula strains were used. The results showed that enzymatically hydrolyzed fat seems to be appropriate source for production of high value biomass. Method of base hydrolyzes proved to be the most efficient for carotenoid production. The composition of cultivation media and cultivation conditions should be further optimized.

Keywords

Carotenoids, Lipides, Yeast, Cultivation

Authors

M. Vysoká, M. Szotkowski, K. Bradáčová, J. Holub, P. Matoušková, S. Šimanský, M. Čertík, I. Márová

Released

18. 5. 2018

ISBN

1336-4839

Periodical

34th Annual Conference on Yeasts, Book of abstracts

State

Slovak Republic

Pages from

Pages to

Pages count

URL

http://yeastconference.sk/wp-content/uploads/2018/05/45th-ANNUAL-CONFERENCEzbornik.pdf

BibTex

@misc{BUT149002,
  author="Petra {Skoumalová} and Marie {Vysoká} and Ivana {Márová} and Martin {Szotkowski} and Kristína {Bradáčová} and Samuel {Šimanský} and Milan {Čertík} and Jiří {Holub}",
  title="BIOCONVERSION OF LOW-COST FAT MATERIALS INTO
HIGH-VALUE PUFA-CAROTENOID-RICH BIOMASS",
  year="2018",
  journal="34th Annual Conference on Yeasts, Book of abstracts",
  pages="64--64",
  issn="1336-4839",
  url="http://yeastconference.sk/wp-content/uploads/2018/05/45th-ANNUAL-CONFERENCEzbornik.pdf",
  note="presentation, poster"
}