Publication detail

High resolution melting analysis for identification of strains beloging to the Lactobacillus casei group

KONEČNÁ, J. CHVALKOVSKÁ, E. KNÁPKOVÁ, M. ŠPANOVÁ, A. RITTICH, B. BRÁZDA, V.

Original Title

Type

conference paper

Language

English

Original Abstract

Molecular methods based on the amplification of 16S ribosomal RNA gene analysis are more rapid, reliable, and reproducible and have greater sensitivity. High resolution melting (HRM) curve analysis is a simple, low-cost, closed tube method for amplicon discrimination and easy connection with real-time polymerase chain reaction (PCR). In this contribution, we report rapid species identification of strains belonging to the Lactobacillus casei group using HRM-PCR. Three different DNA isolation methods were used in this work: phenol extraction, separation using magnetic particles and commercial kit. Two sets of targeted gene fragments primers (groEL, BSH) were tested for amplification of the 16S rRNA gene. Use of groEL primers pair successfully identify strains belong to the Lactobacillus casei group. The variance between used extraction methods for evidence of HRM curves was found.

Keywords

Lactobacillus casei; DNA isolation; High-Resolution Melting Analysis

Authors

KONEČNÁ, J.; CHVALKOVSKÁ, E.; KNÁPKOVÁ, M.; ŠPANOVÁ, A.; RITTICH, B.; BRÁZDA, V.

Released

25. 4. 2018

ISBN

978-80-223-4517-0

Book

Študentská vedecká konferencia PriF UK 2018 Zborník recenzovaných príspevkov

Edition number

první

Pages from

302

Pages to

307

Pages count

1652

BibTex

@inproceedings{BUT147532,
  author="Jana {Konečná} and Eva {Chvalkovská} and Monika {Knápková} and Alena {Španová} and Bohuslav {Rittich} and Václav {Brázda}",
  title="High resolution melting analysis for identification of strains beloging to the Lactobacillus casei group",
  booktitle="Študentská vedecká konferencia PriF UK 2018 Zborník recenzovaných príspevkov",
  year="2018",
  number="první",
  pages="302--307",
  isbn="978-80-223-4517-0"
}