Publication detail

Comparison of extraction techniques for isolation of steroid oestrogens in environmentally relevant concentrations from sediment

SADÍLEK, J. SPÁLOVSKÁ, P. VRANA, B. VÁVROVÁ, M. MARŠÁLEK, B. ŠIMEK, Z.

Original Title

Type

journal article in Web of Science

Language

English

Original Abstract

The comparison of four extraction techniques for isolation of five native and one labelled steroid oestrogens from sediment was described. The three conventional extraction techniques Soxhlet warm extraction (SWE), accelerated solvent extraction (ASE), microwave-assisted extraction (MAE) and a promising technique QuEChERS were tested for isolation of low environmentally relevant oestrogen concentrations using different extraction conditions. The least expensive and time-consuming method QuEChERS provided the best extraction recoveries (53–84%) from all techniques. MAE achieved the highest recovery from conventional techniques for less polar oestrogens using dichloromethane: acetone 3:1 mixture as an extraction solvent (50–71%), but for extraction of the whole group of oestrogens including more polar estriol acetone or methanol must be used. ASE provided higher extraction recoveries using dichloromethane at 60°C (53–74%) for less polar oestrogens. However, the repeatability of results was unsatisfactory and recoveries using other extraction conditions were lower than for MAE. The most time-consuming SWE achieved the worst extraction recoveries and for isolation of low oestrogen concentrations from sediments, it is completely unsuitable.

Keywords

estrogens; sediment; Soxhlet warm extraction (SWE); accelerated solvent extraction (ASE); microwave-assisted extraction (MAE); quick easy cheap effective rugged safe (QuEChERS)

Authors

SADÍLEK, J.; SPÁLOVSKÁ, P.; VRANA, B.; VÁVROVÁ, M.; MARŠÁLEK, B.; ŠIMEK, Z.

Released

15. 9. 2016

ISBN

0306-7319

Periodical

International Journal of Environmental Analytical Chemistry

Year of study

Number

State

United Kingdom of Great Britain and Northern Ireland

Pages from

1022

Pages to

1037

Pages count

BibTex

@article{BUT140720,
  author="Jan {Sadílek} and Petra {Spálovská} and Branislav {Vrana} and Milada {Vávrová} and Blahoslav {Maršálek} and Zdeněk {Šimek}",
  title="Comparison of extraction techniques for isolation of steroid oestrogens in environmentally relevant concentrations from sediment",
  journal="International Journal of Environmental Analytical Chemistry",
  year="2016",
  volume="96",
  number="11",
  pages="1022--1037",
  doi="10.1080/03067319.2016.1232718",
  issn="0306-7319"
}