Publication detail

Application of magnetic polymethacrylate-based microspheres for the isolation of DNA from raw vegetables and processed foods of plant origin

TROJÁNEK, Z. KOVAŘÍK, A. ŠPANOVÁ, A. MAROŠIOVÁ, K. HORÁK, D. RITTICH, B.

Original Title

Type

journal article in Web of Science

Language

English

Original Abstract

Plant samples are generally characterised by a complex composition containing various inhibitors of PCR. Here we applied magnetic hydrophilic P(HEMA-co-GMA) microspheres to extract DNA from raw vegetables and highly processed foods of plant origin. Proposed small scale PCR-ready DNA extraction protocol was comparable with a silica-based DNeasy Plant Mini Kit (Qiagen) and classical CTAB extraction methods in quality and amplificability of DNA while it is less costly and time consuming.

Keywords

raw vegetables; processed foods; DNA isolation; magnetic P(HEMA-co-GMA) microspheres; qPCR

Authors

TROJÁNEK, Z.; KOVAŘÍK, A.; ŠPANOVÁ, A.; MAROŠIOVÁ, K.; HORÁK, D.; RITTICH, B.

Released

5. 1. 2018

Publisher

John Wiley & Sons, Inc.

Location

USA

ISBN

1745-4549

Periodical

Journal of Food Processing and Preservation

Year of study

Number

State

United States of America

Pages from

Pages to

Pages count

BibTex

@article{BUT137030,
  author="TROJÁNEK, Z. and KOVAŘÍK, A. and ŠPANOVÁ, A. and MAROŠIOVÁ, K. and HORÁK, D. and RITTICH, B.",
  title="Application of magnetic polymethacrylate-based microspheres for the isolation of DNA from raw vegetables and processed foods of plant origin",
  journal="Journal of Food Processing and Preservation",
  year="2018",
  volume="42",
  number="1",
  pages="1--8",
  doi="10.1111/jfpp.13384",
  issn="1745-4549"
}