Publication detail

New insights into Pseudomonas fluorescens alginate biosynthesis relevant for the establishment of an efficient production process for microbial alginates.

MALEKI, Susan, Mali MÆRK, Radka HRUDIKOVA, Svein VALLA a Helga ERTESVÅG

Original Title

New insights into Pseudomonas fluorescens alginate biosynthesis relevant for the establishment of an efficient production process for microbial alginates.

Type

journal article in Web of Science

Language

English

Original Abstract

Our group has been studying alginate biosynthesis in Pseudomonas fluorescens using several complementary approaches. Alginate is synthesised and transported out of the cell by a multiprotein complex spanning from the inner to the outer membrane. We have developed an immunogold labelling procedure in which the porin AlgE, as a part of this alginate factory, could be detected by transmission electron microscopy. No time-dependent correlation between the number of such factories on the cell surface and alginate production level was found in alginate-producing strains. Alginate biosynthesis competes with the central carbon metabolism for the key metabolite fructose 6-phosphate. In P. fluorescens, glucose, fructose and glycerol, are metabolised via the Entner-Doudoroff and pentose phosphate pathways. Mutational analysis revealed that disruption of the glucose 6-phosphate dehydrogenase gene zwf-1 resulted in increased alginate production when glycerol was used as carbon source. Furthermore, alginate-producing P. fluorescens strains cultivated on glucose experience acid stress due to the simultaneous production of alginate and gluconate. The combined results from our studies strongly indicate that the availability of fructose 6-phosphate and energy requires more attention in further research aimed at the development of an optimised alginate production process.

Keywords

Alginate; Fructose 6-phosphate; Pseudomonas fluorescens; Transmission electron microscopy; zwf

Authors

MALEKI, Susan, Mali MÆRK, Radka HRUDIKOVA, Svein VALLA a Helga ERTESVÅG

Released

1. 9. 2016

Publisher

New Biotechnology

ISBN

1871-6784

Periodical

New Biotechnology

Year of study

1

Number

32

State

Kingdom of the Netherlands

Pages from

12

Pages to

18

Pages count

6

URL

http://linkinghub.elsevier.com/retrieve/pii/S1871678416323925

BibTex

@article{BUT128471,
  author="Radka {Hrudíková}",
  title="New insights into Pseudomonas fluorescens alginate biosynthesis relevant for the establishment of an efficient production process for microbial alginates.",
  journal="New Biotechnology",
  year="2016",
  volume="1",
  number="32",
  pages="12--18",
  doi="10.1016/j.nbt.2016.08.005",
  issn="1871-6784",
  url="http://linkinghub.elsevier.com/retrieve/pii/S1871678416323925"
}