Publication detail
Fluorescence anisotropy study of microstructural changes of hyaluronan-cetyltrimethylammonium bromide hydrogel
HOLÍNKOVÁ, P. PEKAŘ, M.
Original Title
Fluorescence anisotropy study of microstructural changes of hyaluronan-cetyltrimethylammonium bromide hydrogel
Type
abstract
Language
English
Original Abstract
This fluorescence study deals with the microstructural changes of physical hydrogel formed by mixing hyaluronan solution with oppositely-charged surfactant cetyltrimethylammonium bromide (CTAB), where micelles of CTAB form directly either physical crosslinks of the hyaluronan hydrogel network. Fluorescence measurements were carried out on this hyaluronan-based hydrogels containing two different fluorescence probes, hydrophobic perylene and hydrophilic one, rhodamine 6G. The excitation and emission spectra, fluorescence intensity, steady-state fluorescence anisotropy and lifetime of probes in the samples were measured. The fluorescence anisotropy values obtained in these experiments were used to estimate a microviscosity of the corresponding hydrogel regions during ageing. It is observed that the hydrophobic domains do not undergo significant structural changes. However, the microviscosity of hydrophilic parts gradually decreases, which indicates some structural changes in the nearby area of rhodamine molecules during ageing.
Keywords
hydrogel, hyaluronan, CTAB, microviscosity, fluorescence anisotropy
Authors
HOLÍNKOVÁ, P.; PEKAŘ, M.
Released
11. 9. 2016
Publisher
Leibniz-Institut für Polymerforschung Dresden e. V.
Location
Dresden, Germany
ISBN
978-3-9816007-4-2
Book
20th European Symposium on Polymer Spectroscopy ESOPS 20
Pages from
65
Pages to
65
Pages count
1
BibTex
@misc{BUT128284,
author="Petra {Holínková} and Miloslav {Pekař}",
title="Fluorescence anisotropy study of microstructural changes of hyaluronan-cetyltrimethylammonium bromide hydrogel",
booktitle="20th European Symposium on Polymer Spectroscopy ESOPS 20",
year="2016",
pages="65--65",
publisher="Leibniz-Institut für Polymerforschung Dresden e. V.",
address="Dresden, Germany",
isbn="978-3-9816007-4-2",
note="abstract"
}