Publication detail

Fluorescence study of effect of hyaluronan addition to micelle solution of cetyltrimethylammonium bromide

HOLÍNKOVÁ, P. PEKAŘ, M.

Original Title

Fluorescence study of effect of hyaluronan addition to micelle solution of cetyltrimethylammonium bromide

Type

conference paper

Language

English

Original Abstract

This study deals with the effect of hyaluronan addition on the micellar formation of cationic surfactant cetyltrimethylammonium bromide (CTAB) in aqueous solution. Hyaluronan as negatively charged polyelectrolyte can interact with cationic micelles via electrostatic interactions to form aggregates, which could be potential used for targeted drug delivery. The effect of hyaluronan addition to CTAB solution was study by fluorescence measurement of two fluorescence probes located in different regions of micelles – hydrophobic perylene, which is solubilized in hydrophobic interior and hydrophilic one, fluorescein, which is located on the surface of cationic micelles due to its anionic character. The excitation and emission spectra, intensity of fluorescence, steady-state anisotropy of fluorescence and lifetime of probes in the samples were measured. It is observed, that the addition of hyaluronan to micellar solution of CTAB affected structural changes of microenvironment near the molecules of both fluorescence probes, which are reflected at increasing values of fluorescence anisotropy simultaneously with increasing amount of hyaluronan in solutions.

Keywords

fluorescence anisotropy, micelles, hyaluronan, cetyltrimethylammonium bromide, perylene, fluorescein

Authors

HOLÍNKOVÁ, P.; PEKAŘ, M.

RIV year

2015

Released

3. 12. 2015

Publisher

Vysoké učení technické v Brně, Fakulta chemická, Purkyňova 464/118, 612 00 Brno

Location

Brno

ISBN

978-80-214-5290-9

Book

Studentská odborná konference Chemie je život 2015, Sborník příspěvků

Edition

první

Pages from

244

Pages to

250

Pages count

6

URL

http://www.fch.vut.cz/media/sok/sbornik_prispevku_2015.pdf

BibTex

@inproceedings{BUT126573,
  author="Petra {Holínková} and Miloslav {Pekař}",
  title="Fluorescence study of effect of hyaluronan addition to micelle solution of cetyltrimethylammonium bromide",
  booktitle="Studentská odborná konference Chemie je život 2015, Sborník příspěvků",
  year="2015",
  series="první",
  pages="244--250",
  publisher="Vysoké učení technické v Brně, Fakulta chemická, Purkyňova 464/118, 612 00 Brno",
  address="Brno",
  isbn="978-80-214-5290-9",
  url="http://www.fch.vut.cz/media/sok/sbornik_prispevku_2015.pdf"
}