Publication detail

Formation of G-quadruplex and its utilizing for an automated spectrometric detection of cisplatin

RUTTKAY-NEDECKÝ, B. SKALIČKOVÁ, S. KREMPLOVÁ, M. NEJDL, L. KUDR, J. HYNEK, D. NOVOTNÁ, M. LABUDA, J. ADAM, V. KIZEK, R.

Original Title

Formation of G-quadruplex and its utilizing for an automated spectrometric detection of cisplatin

Type

journal article in Web of Science

Language

English

Original Abstract

Here, we studied the formation of G-quadruplex using spectrophotometric methods (peroxidase activity of G-quadruplex) and verifications of obtained results by voltammetry (cytosine-adenine (CA) or guanine (G) peaks height). The results showed that the peroxidase activity of G-quadruplex increases with the increasing concentrations of K+ ions (1-20 mM) and Na+ ions (1-5 mM) with the highest values observed in the presence of K+ ions. The reverse trend was obtained with NH4+ ions, where the highest peroxidase activity was observed at the lowest concentration of NH4+ ions. The G-quadruplex formation was also proven electrochemically. The addition of 5 mM of K+ ions to the oligonucleotide solution with hemin caused a decrease of both determined peaks. The peroxidase activity of G-quadruplex increased with the increasing concentration of Ag+ ions within the range from 40 nM to 5 mu M. Further, the addition of different concentration of cisplatin (0.5-2 mu M) to the G-quadruplex was monitored. With the addition of cisplatin there was a decrease in peroxidase activity and simultaneously decrease in the CA peak height with the greatest effect in twentieth minute after the addition of cisplatin. The G-quadruplex coupling with gold magnetic nanoparticles was performed and the possibility of automation of spectrophotometric assay was proven

Keywords

G-quadruplex; Silver Ions; Cisplatin; Spectrophotometric Methods; Square Wave Voltammetry; Cyclic Voltammetry

Authors

RUTTKAY-NEDECKÝ, B.; SKALIČKOVÁ, S.; KREMPLOVÁ, M.; NEJDL, L.; KUDR, J.; HYNEK, D.; NOVOTNÁ, M.; LABUDA, J.; ADAM, V.; KIZEK, R.

RIV year

2015

Released

29. 5. 2015

Publisher

ESG

ISBN

1452-3981

Periodical

INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE

Year of study

10

Number

5

State

Republic of Serbia

Pages from

3973

Pages to

3987

Pages count

14

URL

http://www.electrochemsci.org/papers/vol10/100503973.pdf

Full text in the Digital Library

http://hdl.handle.net/11012/201459

BibTex

@article{BUT123366,
  author="Branislav {Ruttkay-Nedecký} and Sylvie {Skaličková} and Monika {Kremplová} and Lukáš {Nejdl} and Jiří {Kudr} and David {Hynek} and Marie {Novotná} and Jan {Labuda} and Vojtěch {Adam} and René {Kizek}",
  title="Formation of G-quadruplex and its utilizing for an automated spectrometric detection of cisplatin",
  journal="INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE",
  year="2015",
  volume="10",
  number="5",
  pages="3973--3987",
  issn="1452-3981",
  url="http://www.electrochemsci.org/papers/vol10/100503973.pdf"
}