Publication detail
SIMPLY METHOD OF DNA ISOLATION FROM LACTIC ACID BACTERIA
FRIČOVÁ, M. ČUTA, R. KŘÍŽOVÁ, J. ŠPANOVÁ, A. RITTICH, B.
Original Title
SIMPLY METHOD OF DNA ISOLATION FROM LACTIC ACID BACTERIA
Type
abstract
Language
English
Original Abstract
Identification of bacterial DNA consists of several steps: cell lysis, extraction and purification of DNA followed by the identification of the bacterial species using polymerase chain reaction (PCR). Genomic DNA was isolated from Lactobacillus plantarum CCM 7039, Lactobacillus fermentum CCM 7192, Lactobacillus rhamnosus CCM 1825, Lactobacillus casei subsp. casei CCM 7088, and Bifidobacterium bifidum CCM 3762 by fenol extraction procedure and by the salting out with NaCl solution. Crude cell lysates were prepared using standard procedure and using of laundry powder. The quality of isolated DNA was verified by UV spectrophotometry and by agarose gel electrophoresis. Isolated DNA was used as a template for PCR
Keywords
lacitc acid bacteria, DNA isolation, salting out procedure, PCR
Authors
FRIČOVÁ, M.; ČUTA, R.; KŘÍŽOVÁ, J.; ŠPANOVÁ, A.; RITTICH, B.
Released
26. 8. 2015
Publisher
Brno University of Technology, Faculty of Chemistry
Location
Brno
ISBN
978-80-214-5228-2
Book
Book of abstracts
Edition
first
Pages from
98
Pages to
99
Pages count
200
BibTex
@misc{BUT116125,
author="FRIČOVÁ, M. and ČUTA, R. and KŘÍŽOVÁ, J. and ŠPANOVÁ, A. and RITTICH, B.",
title="SIMPLY METHOD OF DNA ISOLATION FROM LACTIC ACID BACTERIA",
booktitle="Book of abstracts",
year="2015",
series="first",
volume="2015",
pages="98--99",
publisher="Brno University of Technology, Faculty of Chemistry",
address="Brno",
isbn="978-80-214-5228-2",
note="abstract"
}