Publication detail
Quantitative phase imaging through scattering media by means of coherence-controlled holographic microscope
KOLLÁROVÁ, V. ČOLLÁKOVÁ, J. DOSTÁL, Z. VESELÝ, P. CHMELÍK, R.
Original Title
Quantitative phase imaging through scattering media by means of coherence-controlled holographic microscope
Type
journal article in Web of Science
Language
English
Original Abstract
A coherence-controlled holographic microscope (CCHM) enables quantitative phase imaging with coherent as well as incoherent illumination. The low spatially coherent light induces a coherence gating effect, which makes observation of samples possible also through scattering media. The paper describes theoretically and simulates numerically imaging of a two-dimensional object through a static scattering layer by means of CCHM, with the main focus on the quantitative phase imaging quality. The authors have investigated both strongly and weakly scattering media characterized by different amounts of ballistic and diffuse light. It is demonstrated that the phase information can be revealed also for the case of the static, strongly scattering layer. The dependence of the quality of imaging process on the spatial light coherence is demonstrated. The theoretical calculations and numerical simulations are supported by experimental data gained with a model phase object, as well as living carcinoma cells treated in an optically turbid emulsion
Keywords
quantitative phase imaging; digital holography; microscopy; coherence-controlled holographic microscopy; imaging through scattering media; imaging through turbid media.
Authors
KOLLÁROVÁ, V.; ČOLLÁKOVÁ, J.; DOSTÁL, Z.; VESELÝ, P.; CHMELÍK, R.
RIV year
2015
Released
1. 11. 2015
Publisher
SPIE
ISBN
1083-3668
Periodical
JOURNAL OF BIOMEDICAL OPTICS
Year of study
20
Number
11
State
United States of America
Pages from
1112016-1
Pages to
111206-8
Pages count
8
URL
Full text in the Digital Library