Chitin and chitosan from Brazilian Atlantic Coast: Isolation,characterization and antibacterial activity

journal article in Web of Science

English

Chitin and chitosan were obtained by chemical treatments of shrimp shells. Different particle sizes(50–1000) of the raw material were used to study their effect on size distribution, demineraliza-tion, deproteinization and deacetylation of chitin and chitosan isolation process. The particle size in therange of 800–1000 was selected to isolate chitin, which was achieved by measuring nitrogen, protein, ash, and yield %. Hydrochloric acid (5% v/v) was optimized in demineralization step to remove theminerals from the starting material. Aqueous solution of sodium hydroxide (5 % w/v) at 90 C for (20 h)was used in deproteinization step to remove the protein. Pure chitin was consequently impregnated intohigh concentration of sodium hydroxide (50%) for 3.5 h at 90 C to remove the acetyl groups in order toform high pure chitosan. The degree of deacetylation (DDA) of chitosan was controlled and evaluatedby different analytical tools. The chemical structure of chitin and chitosan was confirmed by elementalanalysis, ATR-FTIR, H-C NMR, XRD, SEM, UV–Vis spectroscopy, TGA, and acid–base titration. The isolatedchitin and chitosan from shrimp shell showed excellent antibacterial activity against Gram bacteria(Escherichia coli) comparing with commercial biopolymers.

Shrimp shells, Chitin, Isolation, Antibacterial activity

ABDEL-RAHMAN, R.; HRDINA, R.; ABDEL-LATTIF, A.; FOUDA, M.; SOLIMAN, A.; MOHAMED, F.; MOHSIN, K.; PINTO, T.

2015

17. 6. 2015

14

0141-8130

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

80

107

Kingdom of the Netherlands

107

120

14