Publication detail
UPLC-MS/MS method for quantification of eight major ergot alkaloids and their corresponding epimers in feed
ČUMOVÁ, M. ČÁSLAVSKÝ, J. KOSUBOVÁ, P., POSPÍCHALOVÁ, M.
Original Title
UPLC-MS/MS method for quantification of eight major ergot alkaloids and their corresponding epimers in feed
Type
conference paper
Language
English
Original Abstract
Ergot alkaloids are mycotoxins produced predominantly by fungi of the Claviceps genus, which mainly affect cereals and wild grasses. Ingestion of ergot alkaloids can cause severe health problems in humans and animals. Thus, there is a need for methods that allow determination of these dangerous toxins. A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed for the simultaneous determination of eight ergot alkaloids: ergosine, ergocornine, ergocryptine and ergocristine and their corresponding epimers as well. Mean recovery, precision, matrix effects and limits of quantification (LOQ) have been assessed for cereal and grass matrices within the method validation. Sample preparation has been based on modified QuEChERS approach. The presented method was used to inspect various feed samples. Ergot alkaloids were found in 31 out of 113 samples investigated. Ergocornine was the most frequently occuring alkaloid, while the highest levels were observed for ergocryptine, ergocristinine and ergosinine, depending on the product type. The total alkaloid content in positive samples varied from 10.4 µg/kg to 1855 µg/kg.
Keywords
alkaloids, ergot alkaloids, feed
Authors
ČUMOVÁ, M.; ČÁSLAVSKÝ, J.; KOSUBOVÁ, P., POSPÍCHALOVÁ, M.
RIV year
2014
Released
6. 12. 2014
Publisher
VUT Brno
Location
Brno
ISBN
978-80-214-5073-8
Book
EMEC15 Book of Abstracts
Pages from
113
Pages to
113
Pages count
523
BibTex
@inproceedings{BUT111924,
author="Martina {Čumová} and Josef {Čáslavský} and Petra {Kosubová}",
title="UPLC-MS/MS method for quantification of eight major ergot alkaloids and their corresponding epimers in feed",
booktitle="EMEC15 Book of Abstracts",
year="2014",
pages="113--113",
publisher="VUT Brno",
address="Brno",
isbn="978-80-214-5073-8"
}