Publication detail
TESTING DIFFERENT TYPES LYSIS METHOD FOR THE PREPARATION OF THE BACTERIAL CELL LYSATES WITH SUBSEQUENT ISOLATION AND AMPLIFICATION OF DNA
Čuta R., Fričová M., Rittich B., Španová A.
Original Title
TESTING DIFFERENT TYPES LYSIS METHOD FOR THE PREPARATION OF THE BACTERIAL CELL LYSATES WITH SUBSEQUENT ISOLATION AND AMPLIFICATION OF DNA
Type
conference proceedings
Language
English
Original Abstract
The aim of this work was the optimization of procedure the crude cell lysates preparation of Lactobacillus genus. In this experiment different lysis methods were tested: buffer B, 4% solution of washing powder Amway and using of immobilized lysozyme. At the same time a lysing reagent was tested. As a control sample was used a buffer A which contained no lysozyme. Crude lysates were used for a DNA isolation using magnetic microspheres. The DNA was examined by the PCR method.
Keywords
DNA, lysis, PCR
Authors
Čuta R., Fričová M., Rittich B., Španová A.
Released
1. 10. 2014
ISBN
978-80-904959-2-0
Pages from
170
Pages to
171
Pages count
2
BibTex
@proceedings{BUT111852,
editor="Robert {Čuta} and Michaela {Čutová} and Bohuslav {Rittich} and Alena {Španová}",
title="TESTING DIFFERENT TYPES LYSIS METHOD FOR THE PREPARATION OF THE BACTERIAL CELL LYSATES WITH SUBSEQUENT ISOLATION AND AMPLIFICATION OF DNA",
year="2014",
pages="170--171",
isbn="978-80-904959-2-0"
}