Publication detail
Use of PCR-DGGE for control of bacteria in chesses and their pickles
ČAKAJDOVÁ, M. TRACHTOVÁ, Š. MOHELSKÝ, T. NĚMEČKOVÁ, I. ŠPANOVÁ, A. RITTICH, B.
Original Title
Use of PCR-DGGE for control of bacteria in chesses and their pickles
Type
conference proceedings
Language
English
Original Abstract
Denaturation gradient gel electrophoresis (PCR-DGGE) was used for control of bacteria in brained cheeses and their pickles. Composition of polymerase chain reaction (PCR) mixture and DNA amplification conditions were optimized for PCR with CG clamp primers. Phenol extraction and magnetic microspheres were used for purification of amplicons. Different numbers and intensities of amplicons reflect different bacterial composition in tested cheeses and pickles.
Keywords
DGGE, cheeses and their pickles
Authors
ČAKAJDOVÁ, M.; TRACHTOVÁ, Š.; MOHELSKÝ, T.; NĚMEČKOVÁ, I.; ŠPANOVÁ, A.; RITTICH, B.
Released
20. 10. 2014
Publisher
Institute of Analytical Chemistry AS CR, v. v. i., Brno
Location
Brno, Czech Republic
ISBN
978-80-904959-2-0
Book
CECE 2014 11th International Interdisciplinary Meeting of Bioanalysis
Pages from
147
Pages to
150
Pages count
4
BibTex
@proceedings{BUT111768,
editor="Martina {Čakajdová} and Štěpánka {Trachtová} and Tomáš {Mohelský} and Irena {Němečková} and Alena {Španová} and Bohuslav {Rittich}",
title="Use of PCR-DGGE for control of bacteria in chesses and their pickles",
year="2014",
pages="147--150",
publisher="Institute of Analytical Chemistry AS CR, v. v. i., Brno",
address="Brno, Czech Republic",
isbn="978-80-904959-2-0"
}