Use high-performance liquid chromatography for determination of saccharides after the enzymatic hydrolysis of waste bread

abstract

English

The main topic of our study was the effect of pH and temperature on the efficiency of enzymatic hydrolysis. For study of enzymatic hydrolysis were used spectrophotometric and HPLC methods. Optimal value of pH and temperature of liquefaction was determined spectrophotometrically by Somogyi-Nelson method. Final yields of glucose from hydrolysis and efficiency of saccharification were determined by HPLC method. As substrate for enzymatic hydrolysis was used waste bread. It was pre-treated by grinding into small particles and then it was made to form 15% w/v suspension. For the hydrolysis have been used two enzymes. For liquefaction of the substrate we used α amylase, and for saccharification we used glucoamylase. It was found that optimal conditions for α amylase were pH 6 and temperature 80 °C. Under these conditions the yield of reducing sugars was 67.08 g∙L-1 (calculated to maltose). Optimal conditions for glucoamylase were pH 4.2 and temperature 60 °C. Obtained yield of glucose was 70.28 g∙L 1.

waste bread, enzymatic hydrolysis, amylases, alpha-amylase, glucoamylase, pH, temperature

HUDEČKOVÁ, H.; ŠUPINOVÁ, P.; BABÁK, L.

2014

20. 10. 2014

Ústav analytické chemie AV ČR, v. v. i.

978-80-904959-2-0

CECE 2014 11th International Interdisciplinary Meeting on Bioanalysis

252

255

475

http://www.ce-ce.org/CECE2014/CECE%202014%20proceedings_full.pdf