Publication detail

An Effect of Cadmium and Lead Ions on Escherichia coli with the ClonedGene for Metallothionein (MT-3) Revealed by Electrochemistry

ADAM, V. CHUDOBOVÁ, D. TMEJOVÁ, K. ČÍHALOVÁ, K. KŘÍŽKOVÁ, S. GURÁŇ, R. KOMÍNKOVÁ, M. ŽŮREK, M. KREMPLOVÁ, M. JIMENEZ JIMENEZ, A. NOVOTNÁ, M. HYNEK, D. PEKAŘÍK, V. KIZEK, R.

Original Title

Type

journal article in Web of Science

Language

English

Original Abstract

This study was focused on the application of electrochemical methods for studying of bacterial strainsEscherichia coli and Escherichia coli expressing human metallothionein gene (MT-3) before and after theapplication of cadmium and/or lead ions in four concentrations (25, 50, 75 and 150 M). Bacterial strainsEscherichia coli and Escherichia coli expressing human metallothionein gene (MT-3) were used like modelorganisms for studying of metals influence to metallothionein expression. Metallothionein was isolatedusing fast protein liquid chromatography and quantified by electrochemical methods. The occurrenceof metallothionein in E.coli was confirmed by gel electrophoresis by the presence of the bands at 15(MT dimer) and 22 kDa (MT trimer).

Keywords

Escherichia coli, Metallothionein, Voltammetry, Cloning, Metal Ionsa

Authors

ADAM, V.; CHUDOBOVÁ, D.; TMEJOVÁ, K.; ČÍHALOVÁ, K.; KŘÍŽKOVÁ, S.; GURÁŇ, R.; KOMÍNKOVÁ, M.; ŽŮREK, M.; KREMPLOVÁ, M.; JIMENEZ JIMENEZ, A.; NOVOTNÁ, M.; HYNEK, D.; PEKAŘÍK, V.; KIZEK, R.

RIV year

2014

Released

10. 9. 2014

ISBN

0013-4686

Periodical

Electrochimica Acta

Year of study

140

Number

State

United Kingdom of Great Britain and Northern Ireland

Pages from

Pages to

Pages count

BibTex

@article{BUT111094,
  author="Vojtěch {Adam} and Dagmar {Hegerová} and Kateřina {Tmejová} and Kristýna {Číhalová} and Soňa {Křížková} and Roman {Guráň} and Markéta {Komínková} and Michal {Žůrek} and Monika {Kremplová} and Ana Maria {Jimenez Jimenez} and Marie {Novotná} and David {Hynek} and Vladimír {Pekařík} and René {Kizek}",
  title="An Effect of Cadmium and Lead Ions on Escherichia coli with the ClonedGene for Metallothionein (MT-3) Revealed by Electrochemistry",
  journal="Electrochimica Acta",
  year="2014",
  volume="140",
  number="SI",
  pages="11--19",
  doi="10.1016/j.electacta.2014.06.091",
  issn="0013-4686"
}