Simple Liquid Chromatography system for Low Flow Rate Gradient Separations

conference paper

English

In this contribution we describe simple approach to the splitless micro-flow liquid chromatograph construction and we demonstrate the functionality of the proposed system. Separation system consists of the programmable syringe pump equipped with the strain gauge force transducer and the high pressure syringe connected to the selector valve via long capillary where mobile phase gradient is generated and stored. Operation of the system is controlled by LabVIEW development environment. Mobile phase gradient is created by successive sucking of defined volumes of four solutions with gradually changing composition into the capillary. Available linear gradients of volumes from 5 to 50 µl traced by uracil are presented. Depending on the selected mode, the system is quickly pressurized and the syringe pump delivers the mobile phase at the constant flow rate or the LabVIEW application drives the syringe pump speed according to the signal from the force transducer and holds the pressure constant. Correct operation of the system was tested by the gradient separation of mixtures of nitro-based explosives. Proper function of the separation system was clearly confirmed by high repeatability of retention times in the constant flow rate mode and by high repeatability of retention volumes in the constant pressure mode (RSD less than 0.5 %).

gradient elution; simple system; low flow rates

ŠESTÁK, J.; KAHLE, V.

2013

4. 12. 2014

Vysoké učení technické v Brně, Fakulta chemická, Purkyňova 464/118, 612 00 Brno

Brno

978-80-214-5078-3

Studentská odborná konference Chemie je život 2014 Sborník příspěvků

první

416

421

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