Publication detail

Remote-controlled robotic platform ORPHEUS as a new tool for detection of bacteria in the environment

NEJDL, L. KUDR, J. ČÍHALOVÁ, K. CHUDOBOVÁ, D. ŽŮREK, M. ŽALUD, L. KOPEČNÝ, L. BURIAN, F. RUTTKAY-NEDECKÝ, B. KŘÍŽKOVÁ, S. NOVOTNÁ, M. HYNEK, D. KOPEL, P. PRÁŠEK, J. ADAM, V. KIZEK, R.

Original Title

Type

journal article in Web of Science

Language

English

Original Abstract

Remote-controlled robotic systems are being used for analysis of various types of analytes in hostile environment including those called extraterrestrial. The aim of our study was to develop a remote-controlled robotic platform (ORPHEUS-HOPE) for bacterial detection. For the platform ORPHEUS-HOPE a 3D printed flow chip was designed and created with a culture chamber with volume 600 L. The flow rate was optimized to 500 L/min. The chip was tested primarily for detection of 1-naphthol by differential pulse voltammetry with detection limit (S/N = 3) as 20 nM. Further, the way how to capture bacteria was optimized. To capture bacterial cells (Staphylococcus aureus), maghemite nanoparticles (1 mg/mL) were prepared and modified with collagen, glucose, graphene, gold, hyaluronic acid, and graphene with gold or graphene with glucose (20 mg/mL). The most up to 50% of the bacteria were captured by graphene nanoparticlesmodified with glucose. The detection limit of the whole assay, which included capturing of bacteria and their detection under remote control operation, was estimated as 30 bacteria per L.

Keywords

Alkaline phosphatase, Bacteria, Electrochemical detection, Magnetic particles, 1-Naphthyl phosphate, Planetary science, Remote sensing

Authors

NEJDL, L.; KUDR, J.; ČÍHALOVÁ, K.; CHUDOBOVÁ, D.; ŽŮREK, M.; ŽALUD, L.; KOPEČNÝ, L.; BURIAN, F.; RUTTKAY-NEDECKÝ, B.; KŘÍŽKOVÁ, S.; NOVOTNÁ, M.; HYNEK, D.; KOPEL, P.; PRÁŠEK, J.; ADAM, V.; KIZEK, R.

RIV year

2014

Released

1. 8. 2014

ISBN

0173-0835

Periodical

Electrophoresis

Year of study

Number

State

Federal Republic of Germany

Pages from

2333

Pages to

2345

Pages count

BibTex

@article{BUT109831,
  author="Lukáš {Nejdl} and Jiří {Kudr} and Kristýna {Číhalová} and Dagmar {Hegerová} and Michal {Žůrek} and Luděk {Žalud} and Lukáš {Kopečný} and František {Burian} and Branislav {Ruttkay-Nedecký} and Soňa {Křížková} and Marie {Novotná} and David {Hynek} and Pavel {Kopel} and Jan {Prášek} and Vojtěch {Adam} and René {Kizek}",
  title="Remote-controlled robotic platform ORPHEUS as a new tool for detection of bacteria in the environment",
  journal="Electrophoresis",
  year="2014",
  volume="35",
  number="14",
  pages="2333--2345",
  doi="10.1002/elps.201300576",
  issn="0173-0835"
}