Publication detail

Self-Assembly Behavior of Negatively Charged Hyaluronan and Oppositely Charged Surfactants

PILGROVÁ, T. PEKAŘ, M.

Original Title

Type

abstract

Language

English

Original Abstract

Physico-chemical interactions between negatively charged hyaluronan and positively charged surfactant result in self-assembly nanoparticles formation. The aim of our study was to prepare and study hyaluronic acid nanoparticles based on electrostatic interactions with oppositely charged surfactant molecules. Interactions of hyaluronan and surfactants were investigated using dynamic light scattering and Laser Doppler Velocimetry methods. Titration experiments (size distribution and zeta potential measurement and isoelectric point) were utilized to explore the formation of aggregates. Aggregates formation is influenced by preparation of system. Different particles are formed if hyaluronan is added to surfactant solution or vice versa. Molecular weights of hyaluronan have inconsiderable effect on interactions of components. If hyaluronan have a role of titrant, final particles are smaller than in the case of surfactant as titrant and can be more suitable for drug delivery applications. Presence of low molecular weight electrolyte in the system has a significant effect on isoelectric point achievement and stability of the system due to reduction of electrostatic repulsion between oppositely charged components. Assuming in the isoelectric point is reached compensation of charges of hyaluronan and surfactant, real degrees of hyaluronan dissociation were determined. Concentration of hyaluronan and conformation of hyaluronan in the system have a significant effect on established degree of dissociation.

Keywords

hylauronan, surfactant, dynamic light scattering, interactions

Authors

PILGROVÁ, T.; PEKAŘ, M.

Released

7. 9. 2014

Location

Limassol, Cyprus, ECIS 2014

Pages from

Pages to

Pages count

BibTex

@misc{BUT109573,
  author="Tereza {Pilgrová} and Miloslav {Pekař}",
  title="Self-Assembly Behavior of Negatively Charged Hyaluronan and Oppositely Charged Surfactants",
  year="2014",
  pages="1",
  address="Limassol, Cyprus, ECIS 2014",
  note="abstract"
}