Publication detail

Identification of Quantum Dots Labeled Metallothionein by Fast Scanning Laser-Induced Breakdown Spectroscopy

NOVOTNÁ, M. NOVOTNÝ, K. KŘÍŽKOVÁ, S. BLAŽKOVÁ, I. KOPEL, P. KAISER, J. HODEK, P. KIZEK, R. ADAM, V.

Original Title

Identification of Quantum Dots Labeled Metallothionein by Fast Scanning Laser-Induced Breakdown Spectroscopy

Type

journal article in Web of Science

Language

English

Original Abstract

The technique described in this paper allows detection of quantum dots (QDs) specifically deposited on the polystyrene surface by laser-induced breakdown spectroscopy (LIBS). Using LIBS, the distribution of QDs or their conjugates with biomolecules deposited on the surface can be observed, regardless of the fact if they exhibit fluorescence or not. QDs deposited on the specific surface of polystyrene microplate in the form of spots are detected by determination of the metal included in the QDs structure. Cd-containing QDs (CdS, CdTe) stabilized with mercaptopropionic (MPA) or ercaptosuccinic (MSA) acid, respectively, alone or in the form of conjugates with metallothionein (MT) biomolecule are determined by using the 508.58 nm Cd emission line. The observed absolute detection limit for Cd in CdTe QDs conjugates with MT in one spot was 3 ng Cd. Due to the high sensitivity of this technique, the immunoanalysis in combination with LIBS was also investigated. Cd spatial distribution in sandwich immunoassay was detected.

Keywords

Laser-induced breakdown spectroscopy; Cadmium; Quantum dots; Metallothionein; Immunoassay

Authors

NOVOTNÁ, M.; NOVOTNÝ, K.; KŘÍŽKOVÁ, S.; BLAŽKOVÁ, I.; KOPEL, P.; KAISER, J.; HODEK, P.; KIZEK, R.; ADAM, V.

RIV year

2014

Released

1. 11. 2014

ISBN

0584-8547

Periodical

Spectrochimica Acta Part B

Year of study

101

Number

11

State

United Kingdom of Great Britain and Northern Ireland

Pages from

10

Pages to

16

Pages count

6

BibTex

@article{BUT109284,
  author="Marie {Novotná} and Karel {Novotný} and Soňa {Křížková} and Iva {Blažková} and Pavel {Kopel} and Jozef {Kaiser} and Petr {Hodek} and René {Kizek} and Vojtěch {Adam}",
  title="Identification of Quantum Dots Labeled Metallothionein by Fast Scanning Laser-Induced Breakdown Spectroscopy",
  journal="Spectrochimica Acta Part B",
  year="2014",
  volume="101",
  number="11",
  pages="10--16",
  doi="10.1016/j.sab.2014.08.037",
  issn="0584-8547"
}