The Study of magnetic P(HEMA-co-GMA) and P(GMA) microspheres using real-time PCR

abstract

English

Isolation of DNA is an important step in the molecular diagnostics of microorganisms and viruses, medical diagnosis, etc. Different magnetic nano- and microparticles have been used as a solid support for DNA isolation. Magnetic nano- and microparticles usually consist of a number of components: supermagnetic core, protective coating and the surface functionality. Iron oxides and some substances used to stabilize magnetic cores reduce biocompatibility of the proposed carriers, therefore perfectly cover core prerequisite for their application in molecular diagnostics. Magnetic cores are covered with a variety of functionalized low-or high-molecular compounds (polymer layer). The work was focused on the estimation of influence of microparticles with magnetite core on the amplification of DNA. A set of 8 types of hydrophilic non-porous poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) – P(HEMA-co-GMA) microparticles, 6 types of poly(glycidyl methacrylate) (PGMA) microparticles and 3 types of individual compounds were studied. The inhibition of DNA amplification was tested by polymerase chain reaction in real time (RT-PCR). The results of study have shown that the real-time PCR can be used for the study of the compatibility of magnetic particles with DNA amplification.

magnetic particles, PCR inhibition

TRACHTOVÁ, Š.; ŠPANOVÁ, A.; HORÁK, D.; RITTICH, B.

20. 7. 2014

Institute of Macromolecular Chemistry, Academy Science of the Czech Republic

Praha, Czech Republic

978-80-85009-80-4

Frontiers of Polymer Colloids: From Synthesis to Macro-Scale and Nano-Scale Applications - Programme Booklet

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