Publication detail

Densitometry and ultrasound velocimetry of hyaluronan solutions in water and in sodium chloride solution

KARGEROVÁ, A. PEKAŘ, M.

Original Title

Type

journal article in Web of Science

Language

English

Original Abstract

The densities of hyaluronan solutions in water and 0.15 M NaCl were measured in the temperature range from 25 to 50 C for the hyaluronan molecular weights from 10 to 1750 kDa. The density increased linearly with concentration and decreased with temperature. The data were fitted by the equation describing the density as a linear function of concentration and a quadratic function of temperature. The effect of molecular weight was negligible and thus single equation was sufficient to describe all data. The apparent and partial specific volumes were calculated from the density data including their extrapolated values to infinite dilutions. The measurement of ultrasound speed in the same solutions under the same conditions enabled to calculate the compressibility and its dependence on concentration and temperature. The compressibility decreased with both the concentration and the temperature but the effect of the concentration was only slight mild. The compressibility was used to estimate the hydration numbers which slightly decreased with increasing temperature and concentration. The addition of NaCl changed only the numerical values of density and ultrasound velocity while not changing the character of their dependence on temperature and concentration. Measured and calculated data indicate that hyaluronan does not disturb the specific water structure in the studied concentration range and support the idea of the existence of water clusters or nanodroplets hydrating the hyaluronan chains in solution.

Keywords

compressibility, density, hyaluronan, hydration, ultrasound velocity

Authors

KARGEROVÁ, A.; PEKAŘ, M.

RIV year

2014

Released

1. 4. 2014

ISBN

0144-8617

Periodical

Carbohydrate Polymers

Year of study

106

Number

State

United Kingdom of Great Britain and Northern Ireland

Pages from

453

Pages to

459

Pages count

BibTex

@article{BUT107460,
  author="Andrea {Hurčíková} and Miloslav {Pekař}",
  title="Densitometry and ultrasound velocimetry of hyaluronan solutions in water and in sodium chloride solution",
  journal="Carbohydrate Polymers",
  year="2014",
  volume="106",
  number="1",
  pages="453--459",
  issn="0144-8617"
}