Publication detail

Application of random mutagenesis to enhance the production of polyhydroxyalkanoates by Cupriavidus necator H16 on waste frying oil

OBRUČA, S. ŠNAJDAR, O. SVOBODA, Z. MÁROVÁ, I.

Original Title

Type

journal article - other

Language

English

Original Abstract

Using random chemical mutagenesis we obtained the mutant of Cupriavidus necator H16 which was capable of improved (about 35 %) production of poly(3-hydroxybuytrate) (PHB) compared to the wild-type strain. The mutant exhibited significantly enhanced specific activities of enzymes involved in oxidative stress response such as malic enzyme, NADP-dependent isocitrate dehydrogenase, glucose-6-phosphate dehydrogenase and glutamate dehydrogenase. Probably, due to the activation of these enzymes, we also observed an increase of NADPH/NADP? ratio. It is likely that as a side effect of the increase of NADPH/NADP ratio the activity of PHBbiosynthetic pathwaywas enhanced,which supported the accumulation of PHB. Furthermore, the mutant was also able to incorporate propionate into copolymer poly(3-hydroxybuytyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] more efficiently than the wild-type strain (Y3HV/prec = 0.17 and 0.29 for the wild-type strain and themutant, respectively)).We assume that it may be caused by lower availability of oxaloacetate for the utilization of propionyl-CoA in 2-methylcitrate cycle due to increased action of malic enzyme. Therefore, propionyl-CoA was incorporated into copolymer rather than transformed to pyruvate via 2-methylcitrate cycle. Thus, the mutant was capable of the utilization of waste frying oils and the production of P(3HB-co-3HV) with better yields and improved content of 3HV resulting in better mechanical properties of copolymer than the wild-type strain. The results of this work may be used for the development of innovative fermentation strategies for the production of PHA and also it might help to define novel targets for the genetic manipulations of PHA producing bacteria.

Keywords

Cupriavidus necator, Polyhydroxyalkanoates, Waste frying oils, Oxidative stress, Random mutagenesis, 2-Methyl citrate cycle

Authors

OBRUČA, S.; ŠNAJDAR, O.; SVOBODA, Z.; MÁROVÁ, I.

RIV year

2013

Released

1. 10. 2013

Publisher

Springer

ISBN

0959-3993

Periodical

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY

Year of study

Number

State

Kingdom of the Netherlands

Pages from

2417

Pages to

2428

Pages count

BibTex

@article{BUT104038,
  author="OBRUČA, S. and ŠNAJDAR, O. and SVOBODA, Z. and MÁROVÁ, I.",
  title="Application of random mutagenesis to enhance the production of polyhydroxyalkanoates by Cupriavidus necator H16 on waste frying oil",
  journal="WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY",
  year="2013",
  volume="29",
  number="12",
  pages="2417--2428",
  doi="10.1007/s11274-013-1410-5",
  issn="0959-3993"
}