Aging of biopolymers and biocolloids studied by light scattering techniques

conference paper

English

Natural product such as biopolymers and biocolloids are often subjected to aggregation and degradation. Both these processes must be taken into account in the case of industrial application of these natural compounds. Aggregated or degraded material as a raw material for industrial production can significantly change the final yield and properties of product. This contribution proposes the application of light scattering techniques for observation of stability of these compounds in time. As model biopolymers were used proteins (bovine serum albumin) and polysaccharides (chitosan, carboxymethylcellulose, glucose, sucrose and sodium alginate). Biocolloids were represented by humic acids. Stability and degradation of proteins and polysaccharides in time was studied using zeta potential (method of electrophoretic light scattering) and molecular weight (combination of size exclusion chromatography with multiangle light scattering detection). Aging of humic acids was investigated by means of zeta potential differences in time and time development of particle size (method of dynamic light scattering). Results showed that the light scattering techniques can be used as easy and quick methods for purposes of detection of the aggregation or degradation of biomaterials. These methods can be useful either in the case of checking of stored stock solutions, for continual controlling of raw materials for industrial production and also for detection of any deviations of final products.

humic acids, light scattering, molecular weight, particle size, polysaccharides, proteins, zeta potential

KALINA, M.; SMILEK, J.; KLUČÁKOVÁ, M.

2013

6. 12. 2013

VUT v Brně

Brno

978-80-214-4823-0

Studentská konference Chemie je život - Sborník příspěvků

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