Publication detail

Analysis of Vitamin D in feed by UPLC-MS/MS

BOLECHOVÁ, M. KOSUBOVÁ, P. NEHYBOVÁ, Z. MRKVICOVÁ, M.

Original Title

Type

conference paper

Language

English

Original Abstract

Various forms of vitamin D, namely cholecalciferol (vitamin D3), ergocalciferol (vitamin D2) and 25-hydroxycholecalciferol, are included in the European Union Register of Feed Additives. Maximum limits for these compounds have been established and vary depending on the feed [1, 2]. The simultaneous use of different forms of vitamin D is prohibited or strictly directed. Central Institute for Supervising and Testing in Agriculture inspects the animal feedstuffs, premixtures and mineral feeds for various parameters including the content of cholecalciferol. Method for determination of vitamin D based on BS EN 12821:2009 [3] has been validated. Sample preparation is based on liquid-liquid extraction after alkaline saponification and determination is carried out by ultra-performance liquid chromatography coupled to tandem mass spectrometry using positive electrospray ionization (UPLC-MS/MS). Deuterated cholecalciferol is used as internal standard for the quantification of cholecalciferol in complex matrices. Since 2012 almost 180 feed samples have been inspected using this method. Feed materials with non-complying contents of Vitamin D were found rarely. The method originally developed for the determination of cholecalciferol was subsequently extended to other fat soluble vitamins and related compounds, such as vitamin A, vitamin E, ergocalciferol, 25-hydroxycholekalciferol, 7-dehydrocholesterol and dihydrotachysterol. Some of these compounds may naturally occur in feed materials and thus may contribute to the total amounts of vitamins in the artificially enriched feeds.Presence of these compounds in feeds and raw materials for feed production has been investigated in order to find out whether the vitamin levels were complying with the declaration values or/and the legislation limits.

Keywords

fat soluble vitamins, vitamin D, chromatography, mass spectrometry

Authors

BOLECHOVÁ, M.; KOSUBOVÁ, P.; NEHYBOVÁ, Z.; MRKVICOVÁ, M.

Released

5. 11. 2013

Publisher

ICT Prague Press

Location

Institute of Chemical Technology, Prague

ISBN

978 80 7080 861 0

Book

6th International Symposium on Recent Advances in Food Analysis

Pages from

215

Pages to

215

Pages count

BibTex

@inproceedings{BUT102653,
  author="BOLECHOVÁ, M. and KOSUBOVÁ, P. and NEHYBOVÁ, Z. and MRKVICOVÁ, M.",
  title="Analysis of Vitamin D in feed by UPLC-MS/MS",
  booktitle="6th International Symposium on Recent Advances in Food Analysis",
  year="2013",
  pages="215--215",
  publisher="ICT Prague Press",
  address="Institute of Chemical Technology, Prague",
  isbn="978 80 7080 861 0"
}