Polygalacturonase immobilized by adsorption on carrier from PET bottles

abstract

English

Polygalacturonase is the part of pectolytic complex which has wide industrial application. By the immobilization of enzyme the improvement of its industrial application properties can be achieved. Commercially used pectolytic complex Rohament P was irreversibly adsorbed on carrier prepared from polyethyleneterephtalate (PET) bottles, what is a new patent of Brno University of Technology. Immobilization of the enzyme resulted in lowering of polygacturonase activity, the measure of which depended on the amount of the enzyme fixed on the carrier. The highest relative activity (96%) had the preparation containing 10 micrograms of the enzyme per 1 g of the carrier. The activity of all preparations was lowered when compared with that of the soluble enzyme. It was dependent on the amount of the enzyme adsorbed on the carrrier. No residual activity of non-adsorbed enzyme was found in any of the solution after a 4 h incubation. Activity of the free and immobilized polygalacturonase was determined at pH 4,2 (0,1 mol/l acetate buffer) and laboratory temperature by measuring the increase of reducing groups during degradation of sodium pectate by a spectrophotometric method. The immobilization led to a partial change of the action pattern onto the high-molecular substrate, manifested in lowering the decrease of viscosity to degradation degree ratio.

polygalacturonase, immobilization, polyethyleneterephtalate, action pattern

ZICHOVÁ, M.; VRÁNOVÁ, D.; OMELKOVÁ, J.; REICHSTÄDTER, M.

2. 10. 2013

University of Ss.Cyril and Methodius in Trnava

Trnava

978-80-8105-501-0

Book of abstracts The 4th International Scientific Conference Applied Natural Sciences 2013 Nový Smokovec, High Tatras, Slovak Republic

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